γ-Glutamyl transpeptidase GGT4 initiates vacuolar degradation of glutathione S-conjugates in Arabidopsis
γ-Glutamyl transpeptidase GGT4 initiates vacuolar degradation of glutathione S-conjugates in Arabidopsis
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DOI：
10.1016/j.febslet.2007.05.071
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发表时间：
2007-07-10
期刊：
影响因子：
3.5
通讯作者：
Meyer, Andreas J.
中科院分区：
文献类型：
--
作者：
Grzam, Anke;Martin, Melinda N.;Meyer, Andreas J.
异源生物质一氯二亚胺在拟南芥的胞质溶胶中与谷胱甘肽缀合，运输至液泡，并水解为半胱氨酸S-二亚胺[Grzam，A.，Tennstedt，P.，Clemens，S.，地狱，R。和Meyer，A. J.（2006）谷胱甘肽S-缀合物的极性螯合胜过植物螯合素合酶对谷胱甘肽部分的可能降解。FEBS Lett. 580，6384-63901。本工作确定γ-谷氨酰转肽酶4（At 4g 29210，GGT 4）作为谷胱甘肽结合物液泡降解的第一步。谷胱甘肽S-二亚胺的水解在A的ggt 4无效突变体中被阻断。thaliana.谷胱甘肽S-二亚胺在突变体中的积累和在野生型植物处理的高亲和力GGT抑制剂acivicin表明，GGT 4是需要启动两步水解序列。GGT 4：绿色荧光蛋白融合物用于证明GGT 4定位于液泡的内腔中。(C)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The xenobiotic monochlorobimane is conjugated to glutathione in the cytosol of Arabidopsis thaliana, transported to the vacuole, and hydrolyzed to cysteine S-bimane [Grzam, A., Tennstedt, P., Clemens, S., Hell, R. and Meyer, A.J. (2006) Vacuolar sequestration of glutathione S-conjugates outcompetes a possible degradation of the glutathione moiety by phytochelatin synthase. FEBS Lett. 580, 6384-63901. The work here identifies gamma-glutamyl transpeptidase 4 (At4g29210, GGT4) as the first step of vacuolar degradation of glutathione conjugates. Hydrolysis of glutathione S-bimane is blocked in ggt4 null mutants of A. thaliana. Accumulation of glutathione S-bimane in mutants and in wild-type plants treated with the high affinity GGT inhibitor acivicin shows that GGT4 is required to initiate the two step hydrolysis sequence. GGT4:green fluorescent protein fusions were used to demonstrate that GGT4 is localized in the lumen of the vacuole. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.