Immune gene transcription in Drosophila adult flies infected by entomopathogenic nematodes and their mutualistic bacteria
Immune gene transcription in Drosophila adult flies infected by entomopathogenic nematodes and their mutualistic bacteria
复制标题
DOI：
10.1016/j.jinsphys.2012.08.003
复制
发表时间：
2013-02-01
影响因子：
2.2
通讯作者：
Eleftherianos, I.
中科院分区：
文献类型：
--
作者：
Castillo, J. C.;Shokal, U.;Eleftherianos, I.
尽管昆虫先天免疫的广泛领域取得了令人瞩目的进展，但我们对昆虫对线虫感染的免疫反应的分子基础的理解仍然不完整。在这里，我们研究了果蝇果蝇中免疫途径基因在昆虫病原性（或昆虫病原性）线虫异杆菌及其共生细菌发光杆菌共同或单独感染后的转录诱导。我们发现，在大多数情况下，野生型成年果蝇感染携带或缺乏共生发光杆菌的异杆线虫会导致 Toll、Imd、JAK/STAT、JNK 和 TGF-β 途径中基因的上调。我们还发现，直接将光杆菌属细菌注射到果蝇体内并不能诱导果蝇抗菌肽基因和应激相关基因的转录。这些结果表明，异杆线虫及其相关的光杆菌采用不同的策略来逃避果蝇免疫反应并建立感染。 (C) 2012 Elsevier Ltd. 保留所有权利。
Despite impressive advances in the broad field of insect innate immunity, our understanding of the molecular basis of insect immune responses to nematode infections remains incomplete. Here we have investigated the transcriptional induction of immune pathway genes in the fruit fly Drosophila melanogaster upon infection with the entomopathogenic (or insect pathogenic) nematodes Heterorhabditis bacteriophora and their mutualistic bacteria Photorhabdus luminescens, either collectively or separately. We show that in most cases, infection of wild-type adult flies with Heterorhabditis nematodes carrying or lacking mutualistic Photorhabdus bacteria results in the up-regulation of genes in the Toll, Imd, JAK/STAT, JNK and TGF-beta pathways. We also find that direct injection of Photorhabdus bacteria into flies fails to induce the transcription of antimicrobial peptide genes and stress-related genes in Drosophila. These results suggest that Heterorhabditis nematodes and their associated Photorhabdus bacteria employ distinct strategies to evade the Drosophila immune response and establish infection. (C) 2012 Elsevier Ltd. All rights reserved.