Brief food restriction increases FA oxidation and glycogen synthesis under insulin-stimulated conditions.
Brief food restriction increases FA oxidation and glycogen synthesis under insulin-stimulated conditions.
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在胰岛素刺激的条件下，短暂的食物限制会增加 FA 氧化和糖原合成。
DOI：
10.1152/ajpregu.00248.2001
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Turcotte,LorraineP
中科院分区：
文献类型：
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作者：
Tucker,MichelleZ;Turcotte,LorraineP
为了确定短暂食物限制对脂肪酸（FA）代谢的影响，在高血糖-高胰岛素条件下（20 mM 葡萄糖、1 mM 棕榈酸盐、1,000 μU/ml 胰岛素、[3-3H]葡萄糖和[1-14C]棕榈酸酯）。与 AL 大鼠相比，FR 大鼠的基础葡萄糖和胰岛素水平显着降低（P<0.05）。 FR 大鼠与 AL 大鼠相比，棕榈酸摄取（34.3 ± 2.7 vs. 24.5 ± 3.1 nmol/g/min）和氧化（3.8 ± 0.2 vs. 2.7 ± 0.3 nmol·g−1·min−1）显着较高（P < 0.05）。 FR 大鼠的葡萄糖摄取增加，并伴随着红腓肠肌和白腓肠肌糖原合成的显着增加，表明胰岛素敏感性的改善。尽管各组间肌肉甘油三酯（TG）水平无显着差异，但葡萄糖摄取量与灌注前总TG浓度呈负相关（r2=0.27，P<0.05）。总之，我们的结果表明，在高血糖-高胰岛素条件下，短暂的 FR 导致 FA 氧化处理增加，这可能有助于改善胰岛素敏感性。
To determine the effects of brief food restriction on fatty acid (FA) metabolism, hindlimbs of F344/BN rats fed either ad libitum (AL) or food restricted (FR) to 60% of baseline food intake for 28 days were perfused under hyperglycemic-hyperinsulinemic conditions (20 mM glucose, 1 mM palmitate, 1,000 μU/ml insulin, [3-3H]glucose, and [1-14C]palmitate). Basal glucose and insulin levels were significantly lower (P< 0.05) in FR vs. AL rats. Palmitate uptake (34.3 ± 2.7 vs. 24.5 ± 3.1 nmol/g/min) and oxidation (3.8 ± 0.2 vs. 2.7 ± 0.3 nmol · g−1· min−1) were significantly higher (P< 0.05) in FR vs. AL rats, respectively. Glucose uptake was increased in FR rats and was accompanied by significant increases in red and white gastrocnemius glycogen synthesis, indicating an improvement in insulin sensitivity. Although muscle triglyceride (TG) levels were not significantly different between groups, glucose uptake and total preperfusion TG concentration were negatively correlated (r2= 0.27,P< 0.05). In conclusion, our results show that under hyperglycemic-hyperinsulinemic conditions, brief FR resulted in an increase in FA oxidative disposal that may contribute to the improvement in insulin sensitivity.