Comparison of DNA marker and pedigree-based methods of genetic analysis of plantain and banana (Musa spp.) clones. I. estimation of genetic relationships
Comparison of DNA marker and pedigree-based methods of genetic analysis of plantain and banana (Musa spp.) clones. I. estimation of genetic relationships
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DNA 标记和基于系谱的车前草和香蕉 (Musa spp.) 克隆遗传分析方法的比较。
DOI：
10.1007/s001220051040
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发表时间：
1999
影响因子：
5.4
通讯作者：
Rodomiro Ortiz
中科院分区：
文献类型：
--
作者：
A. Tenkouano;J. Crouch;H. K. Crouch;D. Vuylsteke;Rodomiro Ortiz
传统的栽培方法对时间和空间的要求都很高。然而，分子遗传学分析的应用可能会显着提高育种效率。本研究的目的是比较估计大蕉和香蕉二倍体、三体和四倍体种质之间代际和代内遗传关系的谱系和DNA标记方法。基于系谱的亲子关系的估计与从分子数据中获得的结果有很大不同。基于标记的贡献的三倍体母体加入到他们的二倍体后代是大于预期的减数分裂模型发表inMusa。相反，母体对四倍体后代的贡献低于预期。基于谱系的相似性是最小的克隆，没有共同的父母和最大的全同胞。基于标记的相似性和系谱关系之间没有关联。虽然DNA标记可以提供一个更准确的描述遗传相关性，这项研究表明，基于谱系的分析可能被证明是有用的选择未来的父母组合在Musceding。
Traditional approaches to the breeding ofMusacrops are highly demanding in terms of both time and space. However, the application of molecular genetic analysis may dramatically improve breeding efficiency. The objectives of the present study were to compare pedigree and DNA marker methods of estimating genetic relationships across and within generations among diploid, triploid and tetraploid accessions of plantain and banana. Pedigree-based estimates of parent-offspring relationships were substantially different from those obtained from molecular data. The marker-based contribution of triploid maternal accessions to their diploid offspring was greater than expected from published models of meiosis inMusa. Conversely, the maternal contribution to tetraploid offspring was less than expected. Pedigree-based similarity was smallest for clones with no common parent and greatest for full-sibs. There was no association between marker-based similarity and pedigree relationships. While DNA markers may provide a more accurate description of genetic relatedness, this study suggests that pedigree-based analysis may prove useful for the selection of prospective parental combinations inMusabreeding.