Fatal hepatorenal failure and thrombocytopenia with SU5416, a vascular endothelial growth factor Flk-1 receptor inhibitor, in AIDS-Kaposi's sarcoma.
Fatal hepatorenal failure and thrombocytopenia with SU5416, a vascular endothelial growth factor Flk-1 receptor inhibitor, in AIDS-Kaposi's sarcoma.
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SU5416（一种血管内皮生长因子 Flk-1 受体抑制剂）在艾滋病-卡波西肉瘤中导致致命性肝肾衰竭和血小板减少症。
DOI：
10.1097/01.aids.0000254688.06831.28
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Bernd,Claus
中科院分区：
文献类型：
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作者：
Noy,Ariela;vonRoenn,Jamie;Politsmakher,Alex;Miles,Steven;Bernd,Claus
在卢旺达基加利进行的一项分析遗传宿主因素对HIV-1垂直传播风险影响的研究中，我们在一名受感染的母亲和她受感染的孩子中发现了CCR 5编码区（表示为hCCR 5D24）中一种新型24 bp缺失的杂合子携带。此外，我们在来自同一地区的36对HIV-1血清不一致夫妇中检测到2例hCCR 5D24杂合子。基加利共有178对HIV-1阳性妇女和儿童参加了2002年3月至2004年10月的一项预防母婴HIV-1传播的队列研究。采集冷冻的外周血单核细胞沉淀，并送至卢森堡的逆转录病毒学实验室，用于分析HIV共受体多态性，包括CCR 5第101位氨基酸的终止密码子（突变m303 [4]）。对于编码hCCR 5D24的样品的测序，使用巢式聚合酶链反应（PCR）方案扩增DNA提取物。使用引物326 F（50-TGCTTGGCCAAAAAGAGAGT-30）和977 R（50-GATGATTCCTGGGAGAGACG-30）进行外部PCR，随后在2%琼脂糖凝胶电泳上分离。使用QiaexII试剂盒（Qiagen，文洛，荷兰）凝胶纯化24 bp缺失的片段，并使用引物472 F（50-GGTGGAACAAGATGGATTATCAAGAGT-30）和882 R（50-CAGCATGGACGACAGCCAGG-30）进行内部PCR。使用BigDye终止子循环测序化学（PE Applied Biosystems，Foster City，加州，USA）和引物472 F和882 R对纯化的扩增子进行测序。
During a study analysing the influence of genetic host factors on the risk of vertical transmission of HIV-1 in Kigali, Rwanda, we identified the heterozygous carriage of a novel 24-bp deletion in the coding region of CCR5 (denoted hCCR5D24) in an infected mother and her infected child. In addition, we detected two subjects heterozygous for hCCR5D24 in a cohort of 36 HIV-1-serodiscordant couples originating from the same region.A total of 178 pairs of HIV-1-positive women and children in Kigali were enrolled in a cohort study for the prevention of mother-to-child HIV-1 transmission, from March 2002 to October 2004. Frozen peripheral blood mononuclear cell pellets were collected and sent to the Retrovirology Laboratory in Luxembourg for the analysis of HIV co-receptor polymorphisms, including a stop codon at amino acid position 101 of CCR5 (mutation m303 [4]). For the sequencing of samples encoding hCCR5D24, DNA extracts were amplified using a nested polymerase chain reaction (PCR) protocol. The outer PCR was performed using primers 326F (50–TGCTTGGCCAAAAAGAGAGT–30) and 977R (50–GATGATTCCTGGGAGAGACG–30) and was subsequently separated on a 2% agarose gel electrophoresis. The 24-bp-deleted fragments were gel-purified using the QiaexII kit (Qiagen, Venlo, The Netherlands) and an inner PCR was performed using primers 472F (50–GGTGGAACAAGATGGATTATCAAGAGT–30) and 882R (50–CAGCATGGACGACAGCCAGG–30). Sequencing was performed on purified amplicons using the BigDye terminator cycle-sequencing chemistry (PE Applied Biosystems, Foster City, California, USA), and primers 472F and 882R.