Lipase-catalyzed transesterification of rapeseed oils for biodiesel production with a novel organic solvent as the reaction medium
Lipase-catalyzed transesterification of rapeseed oils for biodiesel production with a novel organic solvent as the reaction medium
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DOI：
10.1016/j.molcatb.2006.06.012
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发表时间：
2006-12-04
影响因子：
--
通讯作者：
Li, Zebo
中科院分区：
文献类型：
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作者：
Li, Lilin;Du, Wei;Li, Zebo
采用叔丁醇作为一种新型反应介质，通过脂肪酶催化菜籽油酯交换反应制备生物柴油，可以消除甲醇过量和副产物甘油对生物柴油的负面影响。进一步研究了Lipozyme TL IM和Novozym 435联合催化甲醇分解反应的最佳工艺条件：叔丁醇/油体积比1：1，醇油摩尔比4：1，Lipozyme TL IM和Novozym 435质量分数分别为3%和1%，反应温度35 ℃，转速130 rpm，反应时间12 h，生物柴油产率可达95%。以叔丁醇为反应介质，重复使用200次，酶活力无明显损失。此外，还探索了废油用于生物柴油的生产，发现脂肪酶在这种新系统中也表现出良好的稳定性。(c)2006 Elsevier B. V.保留所有权利。
tert-Butanol, as a novel reaction medium, has been adopted for lipase-catalyzed transesterification of rapeseed oil for biodiesel production, with which both the negative effects caused by excessive methanol and by-product glycerol could be eliminated. Combined use of Lipozyme TL IM and Novozym 435 was proposed further to catalyze the methanolysis and the highest biodiesel yield of 95% could be achieved under the optimum conditions (tert-butanol/oil volume ratio 1:1; methanol/oil molar ratio 4:1; 3% Lipozyme TL IM and 1% Novozym 435 based on the oil weight; temperature 35 degrees C; 130 rpm, 12 h). There was no obvious loss in lipase activity even after being repeatedly used for 200 cycles with tert-butanol as the reaction medium. Furthermore, waste oil was also explored for biodiesel production and it has been found that lipase also showed good stability in this novel system. (c) 2006 Elsevier B.V. All rights reserved.