Differences in cell wall components and allocation of boron to cell walls confer variations in sensitivities of Brassica napus cultivars to boron deficiency
Differences in cell wall components and allocation of boron to cell walls confer variations in sensitivities of Brassica napus cultivars to boron deficiency
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DOI：
10.1007/s11104-011-1074-6
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发表时间：
2012-05
期刊：
影响因子：
4.9
通讯作者：
Yuan Pan;Zhenhua Wang;Lu Yang;Zhifang Wang;Lei Shi;R. Naran;P. Azadi;Fangsen Xu
中科院分区：
文献类型：
--
作者：
Yuan Pan;Zhenhua Wang;Lu Yang;Zhifang Wang;Lei Shi;R. Naran;P. Azadi;Fangsen Xu
目的细胞壁是植物体内硼（B）的主要结合位点，不同植物种类对硼的需求量差异由细胞壁中的果胶多糖含量决定。本研究旨在阐明甘蓝型油菜品种B缺陷的细胞壁特性与B分配之间的关系，以及对B缺陷的敏感性差异。方法以两个B效率相反的品种，分析细胞壁果胶含量与糖基组成、B摄取与分配、基因表达和细胞壁超微结构之间的关系。结果甘蓝型油菜B高效品种青优10号对B缺乏的耐受性较高，表现出B缺陷的耐受性。与甘蓝型油菜 B 低效品种 Westar 10 相比，其生物量产量更高，B 缺乏症状更轻，细胞壁增厚更少。这些差异归因于两个因素：首先是在低硼供应情况下，青优10号积累了更多的硼，并且将其分配到细胞壁果胶的比例显着高于西星10号。此外，青优10号细胞壁的B结合位点比Westar 10的细胞壁相对较少，这表明在B缺陷和B充足条件下，细胞壁提取率较低，果胶和糖基残基含量较少。两种条件下 KDOPS 基因表达水平的比较表明，无论 B 水平如何，Westar 10 比青油 10 具有更高的生物合成 B 结合物质的潜力。结论这些结果表明，较高的细胞壁果胶多糖含量以及 B 向细胞壁的有限积累和分配导致 Westar 10 对 B 缺乏的更大敏感性。这两个生理方面可能决定了甘蓝型油菜品种青优10号和威星10号对B缺乏耐受性的差异。相比之下，B在细胞壁中积累和分配的差异对甘蓝型油菜品种对B缺乏的敏感性差异的影响远大于细胞壁成分的影响。
AimsThe cell wall is the main binding site of boron (B) in plants, and the differences in B requirements among different plant species are determined by pectic polysaccharide contents in the cell walls. The aim of this research was to illustrate the relationship between cell wall properties and allocation of B to cell wall and the differential sensitivity ofBrassica napuscultivars to B deficiency.MethodsTwo cultivars with opposite B efficiency were used to analyse the relationship among cell wall pectin contents and glycosyl composition, B uptake and allocation, gene expression and cell wall ultrastructure.ResultsTheBrassica napusB-efficient cultivar Qingyou 10 was more tolerant to B deficiency, exhibiting a higher biomass production, milder B deficiency symptoms and less cell wall thickening compared to theBrassica napusB-inefficient cultivar Westar 10. These differences were attributed to two factors; the first was that Qingyou 10 accumulated more B and distributed significantly higher proportion of it to the cell wall pectins than did Westar 10 under low B supply. Also, the cell walls of Qingyou 10 exhibited relatively less B-binding sites than those of Westar 10, which was indicated by the lower cell wall extraction rates, less pectin and glycosyl residue contents under the B-deficient and B-sufficient conditions. A comparison of theKDOPSgene expression levels in the two conditions suggests that Westar 10 had a higher potential for biosynthesizing B-binding substances than did Qingyou 10, regardless of B levels.ConclusionsThese results suggest that both higher cell wall pectin polysaccharide content, and limited accumulation and allocation of B to the cell walls contribute to the greater sensitivity of Westar 10 to B deficiency. These two physiological aspects may determine the differences in B deficiency tolerance betweenBrassica napuscultivars Qingyou 10 and Westar 10. Comparably, the difference in accumulation and allocation of B to cell wall plays a much more important role than cell wall components to sensitivity difference ofBrassica napuscultivars to B deficiency.