Transcriptional activators in yeast.
Transcriptional activators in yeast.
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DOI：
10.1093/nar/gkj493
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发表时间：
2006
影响因子：
14.9
通讯作者：
Uetz P
中科院分区：
文献类型：
--
作者：
Titz B;Thomas S;Rajagopala SV;Chiba T;Ito T;Uetz P
真核转录激活结构域(ADS)在蛋白质组水平上还没有得到很好的定义。我们利用酵母单杂交系统系统地检测了∼6000酵母蛋白的转录活性，并鉴定了451个转录激活子。然后，我们通过融合Gal4DNA结合域和His3报告基因来确定它们的转录激活强度，His3报告基因包含一个带有Gal4结合位点的启动子。在132个最强的激活因子中，32个是已知的转录因子，另外35个功能未知。尽管锌指、螺旋-环-螺旋结构域和其他几个结构域在激活剂中的表达水平很高，但只有少数几个结构域含有特征性的ADS。我们还发现了一些显著的相关性：激活活性越强，激活剂的酸性越强，富含谷氨酰胺，富含脯氨酸或天冬氨酸。此前已发现约29%的激活子与转录机制特异相互作用，而10%的激活子是转录调控复合体的组成部分。根据它们的转录活性、定位和相互作用模式，至少有6个以前未知的蛋白质被认为是真正的转录调控蛋白(即YFL049W、YJR070C、YDR520C、YGL066W/Sgf73、YKR064W和YCR082W/Ahc2)。
Eukaryotic transcription activation domains (ADs) are not well defined on the proteome scale. We systematicallly tested ∼6000 yeast proteins for transcriptional activity using a yeast one-hybrid system and identified 451 transcriptional activators. We then determined their transcription activation strength using fusions to the Gal4 DNA-binding domain and a His3 reporter gene which contained a promoter with a Gal4-binding site. Among the 132 strongest activators 32 are known transcription factors while another 35 have no known function. Although zinc fingers, helix–loop–helix domains and several other domains are highly overrepresented among the activators, only few contain characterized ADs. We also found some striking correlations: the stronger the activation activity, the more acidic, glutamine-rich, proline-rich or asparagine-rich the activators were. About 29% of the activators have been found previously to specifically interact with the transcription machinery, while 10% are known to be components of transcription regulatory complexes. Based on their transcriptional activity, localization and interaction patterns, at least six previously uncharacterized proteins are suggested to be bona fide transcriptional regulators (namely YFL049W, YJR070C, YDR520C, YGL066W/Sgf73, YKR064W and YCR082W/Ahc2).