Autologous bone marrow implantation induced angiogenesis and improved deteriorated exercise capacity in a rat ischemic hindlimb model
Autologous bone marrow implantation induced angiogenesis and improved deteriorated exercise capacity in a rat ischemic hindlimb model
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DOI：
10.1006/jsre.2000.6080
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发表时间：
2001-04-01
影响因子：
2.2
通讯作者：
Esato, K
中科院分区：
文献类型：
--
作者：
Ikenaga, S;Hamano, K;Esato, K
背景。骨髓拥有内皮祖细胞，可分泌多种生长因子，有助于新毛细血管的形成。在本研究中，我们研究了在大鼠缺血后肢模型中植入自体骨髓细胞（BMC）诱导的血管生成程度，并研究增加的侧支血管是否可以改善恶化的身体功能。材料和方法。通过结扎 Dark Agouti (DA) 大鼠的股动脉及其分支来产生缺血性后肢。 BMC (1 x 10(7)) 在六个点经皮注射到腓肠肌中。为了评估血管生成，在结扎后两周进行组织学评估和微血管造影。通过使用非放射性彩色微球测量内收肌和腓肠肌中的血流量并通过确定结扎后 2 周的股动脉动静脉氧差 (AVDO(2)) 来评估缺血性损伤的严重程度。在电动跑步机上的跑步时间用于代表运动能力。结果。组织学评估和显微血管造影表明植入的BMCs诱导血管生成。与对照组相比，骨髓细胞植入（BMI）组治疗侧内收肌的血流量显着恢复至正常灌注肢体血流量的77.3±19.3%（P < 0.05）。与对照组的AVDO(2)相比，BMI组的AVDO(2)显着下降。结扎后 2 周和 4 周时，BMI 组大鼠的跑步时间大约是对照组大鼠的 1.5 倍（P < 0.01）。结论。在我们的大鼠缺血后肢模型中，自体 BMC 的植入诱导了血管生成并改善了恶化的运动能力。 (C) 2001 年学术出版社。
Background. Bone marrow possesses endothelial progenitor cells that secrete several growth factors and can contribute to the formation of new capillaries. In the present study, we investigated the extent of angiogenesis induced by implantation of autologous bone marrow cells (BMCs) in a rat ischemic hindlimb model and studied whether the increased collateral vessels can improve deteriorated physical function.Materials and methods. Ischemic hindlimb was created by ligation of the femoral artery and its branches in Dark Agouti (DA) rats. BMCs (1 x 10(7)) were injected percutaneously at six points into the gastrocnemius muscle. To assess angiogenesis, histologic evaluation and microangiography were performed at 2 weeks postligation. Severity of the ischemic insult was evaluated by measuring blood flow in the adductor and gastrocnemius muscles using nonradioactive colored microspheres and by determining the femoral arteriovenous oxygen difference (AVDO(2)) at 2 weeks postligation. Running time on a motor-driven treadmill was used to represent exercise capacity.Results. The histologic evaluation and microangiogram showed that the implanted BMCs induce angiogenesis. Blood flow to the adductor muscle on the treated side in the bone marrow cell implantation (BMI) group was significantly restored to 77.3 +/- 19.3% of that of the normally perfused limb in comparison to that in control groups (P < 0.05). AVDO(2) in the BMI group significantly decreased when compared with AVDO(2) in control groups. Rats in the BMI group ran approximately 1.5 times longer than rats in control groups at 2 and 4 weeks postligation (P < 0.01).Conclusions. Implantation of autologous BMCs induced angiogenesis and improved deteriorated exercise capacity in our rat ischemic hindlimb model. (C) 2001 Academic Press.