Bidirectional surface analysis of monomolecular membrane harboring nanoscale reversible collapse structures.

Bidirectional surface analysis of monomolecular membrane harboring nanoscale reversible collapse structures.
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具有纳米级可逆塌陷结构的单分子膜的双向表面分析

DOI:
10.1021/nl303928m
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发表时间:
2013
期刊:
影响因子:
10.8
通讯作者:
H.-J. Galla
H.-J. Galla
中科院分区:
材料科学1区
文献类型:
--
作者:
A.K. Sachan;H.-J. Galla

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在空气-水界面处的朗缪尔膜内形成的纳米级塌陷结构的取向的确定通过现有的实验技术是不可能的。然而,这对于肺表面活性物质膜特别重要,其在动态侧向压缩和扩张下形成可逆的表面相关储库(SAR)。迄今为止,这些SAR相对于界面的方向仍然不确定。我们设计了一种方法来研究在肺表面活性物质衬里的功能类似物中形成的SAR的方向性,在那里我们将压缩膜转移到多孔基底上,并对携带SAR的跨孔单分子膜进行双向表面成像。这清楚地表明了SAR与朝向空气侧的膜的关联,与迄今为止普遍接受的朝向水相的取向的观点相反。
Determination of orientation of nanoscale collapse structures formed within a Langmuir film at the air–aqueous interface has not been possible by existing experimental techniques. This is however of special importance for pulmonary surfactant films, which form reversible surface-associated reservoirs (SARs) under dynamic lateral compression and expansion. The direction of these SARs with respect to the interface has hitherto remained uncertain. We designed a methodological approach to investigate the directionality of SARs formed in a functional analogue of the pulmonary surfactant lining, where we transferred the compressed film on a holey substrate and performed bidirectional surface imaging of the hole spanning monomolecular membrane harboring SARs. This unambiguously showed association of SARs with the membrane toward the air-side, in contrast to the up to now commonly accepted view of an orientation toward the aqueous phase.
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