Fibroblast Growth Factor-10 (FGF-10) Mobilizes Lung-resident Mesenchymal Stem Cells and Protects Against Acute Lung Injury.
Fibroblast Growth Factor-10 (FGF-10) Mobilizes Lung-resident Mesenchymal Stem Cells and Protects Against Acute Lung Injury.
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成纤维细胞生长因子-10 (FGF-10) 动员肺驻留间充质干细胞并预防急性肺损伤
DOI：
10.1038/srep21642
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发表时间：
2016-02-12
影响因子：
4.6
通讯作者：
Song Y
中科院分区：
文献类型：
--
作者：
Tong L;Zhou J;Rong L;Seeley EJ;Pan J;Zhu X;Liu J;Wang Q;Tang X;Qu J;Bai C;Song Y
FGF-10可以预防或减少由于创伤性或感染性肺损伤引起的肺特异性炎症。然而，确切的机制是不好的特点。此外，尚未研究FGF-10对肺驻留间充质干细胞（LR-MSC）的影响。为了更好地表征FGF-10对LR-MSC的作用，将FGF-10经气管内递送到大鼠的肺中。滴注后3天，进行支气管肺泡灌洗，培养塑料粘附细胞，表征，然后在LPS气管内滴注后向大鼠递送治疗。FGF-10动员和培养的细胞的免疫表型分析显示MSC标志物CD 29、CD 73、CD 90和CD 105的表达，以及造血谱系标志物CD 34和CD 45的缺失。这些细胞的多能性通过它们分化成骨细胞、脂肪细胞和软骨细胞的能力来证明。与对照大鼠相比，LPS损伤后将LR-MSC递送到肺中减少了炎症反应，如通过降低的湿干比、减少的中性粒细胞和白细胞募集以及减少的炎性细胞因子所证明的。最后，在大鼠肺中直接递送FGF-10导致经处理的肺中LR-MSC的增加，这表明FGF-10的保护作用可能部分通过肺中LR-MSC的动员来介导。
FGF-10 can prevent or reduce lung specific inflammation due to traumatic or infectious lung injury. However, the exact mechanisms are poorly characterized. Additionally, the effect of FGF-10 on lung-resident mesenchymal stem cells (LR-MSCs) has not been studied. To better characterize the effect of FGF-10 on LR-MSCs, FGF-10 was intratracheally delivered into the lungs of rats. Three days after instillation, bronchoalveolar lavage was performed and plastic-adherent cells were cultured, characterized and then delivered therapeutically to rats after LPS intratracheal instillation. Immunophenotyping analysis of FGF-10 mobilized and cultured cells revealed expression of the MSC markers CD29, CD73, CD90, and CD105, and the absence of the hematopoietic lineage markers CD34 and CD45. Multipotency of these cells was demonstrated by their capacity to differentiate into osteocytes, adipocytes, and chondrocytes. Delivery of LR-MSCs into the lungs after LPS injury reduced the inflammatory response as evidenced by decreased wet-to-dry ratio, reduced neutrophil and leukocyte recruitment and decreased inflammatory cytokines compared to control rats. Lastly, direct delivery of FGF-10 in the lungs of rats led to an increase of LR-MSCs in the treated lungs, suggesting that the protective effect of FGF-10 might be mediated, in part, by the mobilization of LR-MSCs in lungs.