Expressions of P-glycoprotein and multidrug resistance-associated protein correlate with chemosensitivity in vitro in gastric cancer
Expressions of P-glycoprotein and multidrug resistance-associated protein correlate with chemosensitivity in vitro in gastric cancer
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发表时间：
2005
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通讯作者：
Xu Hon
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作者：
Xu Hon
目的：探讨P蛋白的表达；糖蛋白（P;gp）和多药耐药；胃癌组织中相关蛋白（MRP）的表达及其对主要培养的胃癌细胞对阿霉素（ADM）、丝裂霉素化疗敏感性的影响；C (mmc), 5；氟尿嘧啶（5；FU）、顺铂（DDP）、依托泊苷（VP;16）、甲氨蝶呤（MTX）、10、羟campothecine（HCPT）。方法：P的表达式；应用单克隆抗体对48例胃癌石蜡包埋组织的gp和MRP进行免疫组化检测。用MTT法检测胃癌组织对7种抗肿瘤药物的化学敏感性。结果：P阳性率；gp和MRP的共表达率分别为41.7%和29.2%，共表达率为25.0%。癌组织和癌旁组织中表达率和表达强度无显著差异。7种抗肿瘤药物中，ADM、VP；16， HCPT显著提高P的表达率；gp， MRP，以及它们的共表达率比化学敏感性高。结论：P阳性表达；gp和MRP可导致原发性化疗耐药。P介导的MDR；gp和MRP是影响ADM、VP耐药的重要因素；16、HCPT。
Objective:To explore the expressions of P;glycoprotein (P;gp) and multidrug resistance;associated protein (MRP) in tissues of gastric cancers and their effect on chemosensitivity of gastric cancer cells cultured primarily to adriamycin (ADM), mitomycin;C (MMC), 5;fluorouracil (5;FU), cisplatin (DDP), etoposide(VP;16), methotrexate(MTX), 10;hydroxycampothecine(HCPT). Methods: The expressions of P;gp and MRP in 48 cases of paraffin embedded gastric cancer tissues were detected immunohistochemically used monoclonal antibodies. The chemosensitivity of the gastric cancer tissues to the seven antitumor agents obtained by MTT assay through primary cultured cells from excised specimens were examined. Results: The positive rate of P;gp and MRP was 41.7% and 29.2%, respectively, and the coexpression rate was 25.0%. There were no significant difference of expression rate and strength in cancer tissue and adjacent tissue. Of the seven antitumor agents, ADM, VP;16, and HCPT had significantly increased expression rates of P;gp, MRP, as well as their coexpression rates than chemosensitivity ones. Conclusions: The positive expression of P;gp and MRP can result in primary chemoresistance. The MDR mediated by P;gp and MRP is an important factor for chemoresistance of ADM, VP;16, and HCPT.