Molecular cloning and expression analysis of tea plant aquaporin (AQP) gene family.
Molecular cloning and expression analysis of tea plant aquaporin (AQP) gene family.
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DOI：
10.1016/j.plaphy.2014.07.011
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发表时间：
2014-10
期刊：
影响因子：
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通讯作者：
Chuan Yue;H. Cao;Lu Wang;Yanhua Zhou;Xinyuan Hao;Jian-ming Zeng;Xinchao Wang;Yajun Yang
中科院分区：
文献类型：
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作者：
Chuan Yue;H. Cao;Lu Wang;Yanhua Zhou;Xinyuan Hao;Jian-ming Zeng;Xinchao Wang;Yajun Yang
水通道蛋白（AQP）在植物中的作用已被广泛研究。然而，茶树（Camellia sinensis）水通道蛋白的信息尚不清楚。本研究从茶树中分离到20个全长AQPcDNA序列，并将其分为5个亚科。这些亚家族中的基因在所研究的组织中显示出差异表达谱。CsAQP的表达模式与花的发育开放（FDO）和芽的内休眠（BED）有关。为了更好地了解CsAQPsin在非生物胁迫下的短期表达模式，分别用脱落酸（阿坝）、冷、盐和干旱处理茶树。阿坝处理可下调多种CsAQP的表达。盐可上调大多数CsAQP基因的转录。冷处理导致了各种CsAQP复杂的转录调控模式。干旱诱导叶片中CsAQP的表达，尤其是质膜内在蛋白（CsPIPs）和液泡膜内在蛋白（CsTIPs）的表达，复水后叶片中的表达仍相对较高，而根中几乎所有的CsAQP均被抑制。我们的研究结果突出了茶树中CsAQPs的多样性，并证明CsPIP和CsTIP基因在胁迫反应以及FDO和BED中起着至关重要的作用。此外，某些CsSIP（small basic intrinsic proteins）、CsNIPs（NOD 26-like intrinsic proteins）和CsXIPs（X intrinsic proteins）可能参与BED和FDO的调控。
The role of aquaporin proteins (AQPs) has been extensively studied in plants. However, the information of AQPs in the tea plant (Camellia sinensis) is unclear. In this manuscript, we isolated 20 full-lengthAQPcDNAs from the tea plant, and these sequences were classified into five subfamilies. The genes in these subfamilies displayed differential expression profiles in the studied tissues. TheCsAQPexpression patterns correlated with flower development and opening (FDO) and bud endodormancy (BED). To better understand the short-term expression patterns ofCsAQPsin response to abiotic stress, tea plants were treated with abscisic acid (ABA), cold, salt or drought. ABA treatment down-regulated the expression of variousCsAQPs. Salt up-regulated the transcription of mostCsAQPgenes. Cold treatment resulted in a complicated transcriptional regulation pattern for variousCsAQPs. The expression ofCsAQPs, especially plasma membrane intrinsic proteins (CsPIPs) and tonoplast intrinsic proteins (CsTIPs), was induced by drought and remained relatively high after rehydration in leaves, whereas almost all theCsAQPswere repressed in roots. Our results highlighted the diversity ofCsAQPsin the tea plant and demonstrated that theCsPIPandCsTIPgenes play a vital role in the stress response as well as in FDO and BED. Furthermore, certainCsSIPs(small basic intrinsic proteins),CsNIPs(NOD26-like intrinsic proteins) andCsXIPs(X intrinsic proteins) may regulate BED and FDO.