Lanthanide ions as versatile catalyst in biochemistry: Efficient site-selective scission of RNA by free lanthanide ions
Lanthanide ions as versatile catalyst in biochemistry: Efficient site-selective scission of RNA by free lanthanide ions
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DOI：
10.1016/j.jallcom.2004.12.149
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发表时间：
2006-02
影响因子：
6.2
通讯作者：
A. Kuzuya;Kenzo Machida;T. Sasayama;Yun Shi;R. Mizoguchi;M. Komiyama
中科院分区：
文献类型：
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作者：
A. Kuzuya;Kenzo Machida;T. Sasayama;Yun Shi;R. Mizoguchi;M. Komiyama
通过将镧系元素（III）离子与带有吖啶的寡核苷酸结合来制备用于位点选择性RNA切断的新系统。修饰的寡核苷酸与底物RNA形成异源双链体，并选择性地激活吖啶前面的磷酸二酯键。因此，这些键比其他键优先水解，即使镧系元素（III）离子没有固定在任何地方。在生理条件下，无论靶位点的序列如何，切割都是有效的。强调了镧系元素的种类对断裂的影响。
Novel systems for site-selective RNA scission are prepared by combining lanthanide(III) ions with oligonucleotides bearing an acridine. The modified oligonucleotide forms a heteroduplex with the substrate RNA, and selectively activates the phosphodiester linkages in front of the acridine. As a result, these linkages are preferentially hydrolyzed over the others, even though the lanthanide(III) ions are not fixed anywhere. The scission is efficient under physiological conditions, irrespective of the sequence at the target site. Effect of the kind of the lanthanide on the scission is emphasized.