In vivo imaging of changes in tumor oxygenation during growth and after treatment
In vivo imaging of changes in tumor oxygenation during growth and after treatment
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DOI：
10.1002/mrm.21212
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发表时间：
2007-05-01
影响因子：
3.3
通讯作者：
Kuppusamy, Periannan
中科院分区：
文献类型：
--
作者：
Bratasz, Anna;Pandian, Ramasamy P.;Kuppusamy, Periannan
报道了一种新的在体成像植入肿瘤中氧分压（pO（2））的方法。该程序使用嵌入肿瘤细胞中的氧传感纳米探针的电子顺磁共振成像（EPRI）。与现有的PO 2定量方法不同，其中探针在测量时被物理插入，新方法使用用氧传感探针预内化（标记）的细胞，这些细胞永久嵌入发育的肿瘤中。放射诱导的纤维肉瘤（RIF-1）细胞，内化与纳米探针的锂八正丁氧基萘（LiNc-BuO），允许生长为实体瘤。生长肿瘤的体内成像显示自旋探针的不均匀分布以及肿瘤体积中的氧合。在肿瘤暴露于单剂量30-戈伊X射线后获得的PO 2图像显示出明显的再分布以及组织氧合的总体增加，在照射后6小时最大增加。然而，较大的肿瘤与灌注差的核心没有显着的变化，氧合。总之，使用具有嵌入式氧敏感纳米探针的体内EPR技术使得能够非侵入性地可视化生长和辐照的肿瘤的细胞内PO 2的动态变化。
A novel procedure for in vivo imaging of the oxygen partial pressure (pO(2)) in implanted tumors is reported. The procedure uses electron paramagnetic resonance imaging (EPRI) of oxygen-sensing nanoprobes embedded in the tumor cells. Unlike existing methods Of PO2 quantification, wherein the probes are physically inserted at the time of measurement, the new approach uses cells that are preinternalized (labeled) with the oxygen-sensing probes, which become permanently embedded in the developed tumor. Radiation-induced fibrosarcoma (RIF-1) cells, internalized with nanoprobes of lithium octa-n-butoxy-naphthalocyanine (LiNc-BuO), were allowed to grow as a solid tumor. In vivo imaging of the growing tumor showed a heterogeneous distribution of the spin probe, as well as oxygenation in the tumor volume. The PO2 images obtained after the tumors were exposed to a single dose of 30-Gy X-radiation showed marked redistribution as well as an overall increase in tissue oxygenation, with a maximum increase 6 hr after irradiation. However, larger tumors with a poorly perfused core showed no significant changes in oxygenation. In summary, the use of in vivo EPR technology with embedded oxygen- sensitive nanoprobes enabled noninvasive visualization of dynamic changes in the intracellular PO2 of growing and irradiated tumors.