Anti-inflammatory properties of enamel matrix derivative in human blood
Anti-inflammatory properties of enamel matrix derivative in human blood
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DOI：
10.1111/j.1600-0765.2005.00863.x
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发表时间：
2006-06-01
影响因子：
3.5
通讯作者：
Aasen, AO
中科院分区：
文献类型：
--
作者：
Myhre, AE;Lyngstadaas, SP;Aasen, AO
从猪牙芽中提取的牙釉质基质衍生物（EMD）已被证明可促进严重牙周炎患者的牙周愈合。这涉及炎症反应的调节，随后是牙周再生的开始。基于这些观察，我们检测了EMD调节促炎细胞因子[肿瘤坏死因子（TNF）-α]释放的能力，抗炎细胞因子（白细胞介素-10）和趋化因子（白细胞介素-8）来自健康供体的全血被脂多糖或肽聚糖攻击，并与不同浓度的EMD或cAMP类似物8-（4-氯苯基）硫代-cAMP（8-CPT-cAMP）。酶联免疫吸附试验（ELISA）检测血浆中TNF-α、IL-8和IL-10，酶免疫分析（EIA）检测外周血单个核细胞裂解液中cAMP水平，结果表明EMD可抑制脂多糖或肽聚糖刺激的健康献血者全血中TNF-α和IL-8的释放，而IL-10的释放无明显变化。牙釉质基质衍生物也产生了四倍增加的cAMP水平的外周血单核细胞裂解物。与EMD一样，8-CPT-cAMP减弱了脂多糖激发的血液中TNF-α的形成，但不减弱白细胞介素-10的形成。釉质基质衍生物限制了人血液中脂多糖或肽聚糖诱导的促炎细胞因子的释放，表明其具有抗炎特性。我们建议，EMD的这种效果是，至少部分地，继发于外周血单核细胞中cAMP的细胞内水平的增加。
Enamel matrix derivative (EMD), extracted from porcine tooth buds, has been shown to promote periodontal healing in patients with severe periodontitis. This involves modulation of the inflammatory response followed by the onset of periodontal regeneration. Based on these observations, we examined the ability of EMD to modulate the release of a pro-inflammatory cytokine [tumor necrosis factor (TNF)-alpha], an anti-inflammatory cytokine (interleukin-10) and a chemokine (interleukin- 8) in whole human blood challenged by bacterial cell wall components.Whole blood from healthy donors was challenged by lipopolysaccharide or peptidoglycan and incubated with different concentrations of EMD or a cAMP analogue 8-(4-chlorophenyl)thio-cAMP (8-CPT-cAMP). TNF-alpha, interleukin-8 and interleukin-10 were analysed from plasma by enzyme-linked immunosorbent assay (ELISA) while cAMP levels of peripheral blood mononuclear cell lysates were analysed by enzyme immunoassay (EIA).We found that EMD attenuated the release of TNF-alpha and interleukin-8 in whole blood from healthy donors challenged by lipopolysaccharide or peptidoglycan, while the release of interleukin-10 was unchanged. Enamel matrix derivative also produced a four-fold increase in the cAMP levels of peripheral blood mononuclear cell lysates. Like EMD, 8-CPT-cAMP attenuated the formation of TNF-alpha, but not of interleukin-10, in blood challenged by lipopolysaccharide.Enamel matrix derivative limits the release of pro-inflammatory cytokines induced by lipopolysaccharide or peptidoglycan in human blood, suggesting that it has anti-inflammatory properties. We propose that this effect of EMD is, at least partly, secondary to an increase in the intracellular levels of cAMP in peripheral blood mononuclear cells.