The transcriptional coactivators p300 and CBP are histone acetyltransferases
The transcriptional coactivators p300 and CBP are histone acetyltransferases
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DOI：
10.1016/s0092-8674(00)82001-2
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发表时间：
1996-11-29
期刊：
影响因子：
64.5
通讯作者：
Nakatani, Y
中科院分区：
文献类型：
--
作者：
Ogryzko, VV;Schiltz, RL;Nakatani, Y
P300/CBP是一种转录适配子，通过直接相互作用整合来自许多序列特异性激活子的信号。各种细胞和病毒因子以p300/CBP为靶点，调节转录和/或细胞周期进程。其中，细胞内的p300/CBP相关因子(PCAF)具有内在的组蛋白乙酰转移酶活性。在这里，我们证明了p300/CBP不仅是一个转录接头，而且是一个组蛋白乙酰转移酶。P300/CBP代表了一类新的乙酰转移酶，因为它没有在其他各种乙酰转移酶中发现的保守基序。P300/CBP乙酰化核小体中的所有四个核心组蛋白。这些观察结果表明，p300/CBP乙酰化核小体与PCAF一致。
p300/CBP is a transcriptional adaptor that integrates signals from many sequence-specific activators via direct interactions. Various cellular and viral factors target p300/CBP to modulate transcription and/or cell cycle progression. One such factor, the cellular p300/CBP associated factor (PCAF), possesses intrinsic histone acetyltransferase activity. Here, we demonstrate that p300/CBP is not only a transcriptional adaptor but also a histone acetyltransferase. p300/CBP represents a novel class of acetyltransferases in that it does not have the conserved motif found among various other acetyltransferases. p300/CBP acetylates all four core histones in nucleosomes. These observations suggest that p300/CBP acetylates nucleosomes in concert with PCAF.