Identification and genetic characterization of two porcine astroviruses from domestic piglets in China
Identification and genetic characterization of two porcine astroviruses from domestic piglets in China
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DOI：
10.1007/s00705-015-2569-x
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发表时间：
2015-12-01
影响因子：
2.7
通讯作者：
Duan, Zhao-jun
中科院分区：
文献类型：
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作者：
Li, Jin-song;Li, Mao-zhong;Duan, Zhao-jun
猪星状病毒分为五种基因型。在这项研究中，我们通过对 15 日龄以下健康家猪的粪便样本使用序列独立单引物扩增 (SISPA) 鉴定了两种猪星状病毒（AstV-LL-1 和 AstV-LL-2）。两者的检出率为 2.82% (14/497)。然后对 AstV-LL 进行测序和表征。系统发育分析表明，它们具有猪星状病毒（PastV）2和5的特征，并具有一些独特的遗传特征。我们的研究结果表明，这两种星状病毒是PAstV2和5的新谱系。这些发现可能有助于评估猪星状病毒的生态和进化。
Porcine astrovirus are divided into five genotypes. In this study, we identified two porcine astroviruses (AstV-LL-1 and AstV-LL-2) by using sequence-independent single-primer amplification (SISPA) on faecal specimens of healthy domestic piglets younger than 15 days. The detection rate for both was 2.82 % (14/497). AstV-LLs were then sequenced and characterised. Phylogenetic analysis revealed that they have the characteristics of porcine astrovirus (PastV) 2 and 5 and have some unique genetic features. Our findings show that the two astroviruses are novel lineages of PAstV2 and 5. The findings may be helpful in evaluating the ecology and evolution of astroviruses in pigs.