Effects of single P-element insertions on bristle number and viability in Drosophila melanogaster.
Effects of single P-element insertions on bristle number and viability in Drosophila melanogaster.
复制标题
单个 P 元件插入对果蝇刚毛数量和活力的影响。
DOI：
10.1093/genetics/143.1.277
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Mackay,TF
中科院分区：
文献类型：
--
作者：
Lyman,RF;Lawrence,F;Nuzhdin,SV;Mackay,TF
在无P元件的等基因背景下，用单P元件诱变方法，在三条主染色体上构建了1094个P [1ArB]插入株系。通过与392个对照系进行比较，评估了插入对刚毛数量和生存能力的影响。P-元件插入片段对猪鬃数和生活力的影响和方差均大于自发突变的影响。刷毛数量的影响的分布是对称的，高度尖峰，这样的几个插入大的影响造成了大部分的方差增加。对生存力的影响呈负偏态和平顶峰分布。平均而言，P元素插入对猪鬃数的影响为部分隐性，对生活力的影响为完全隐性，对猪鬃数影响大的P元素插入物有降低生活力的趋势，但对猪鬃数影响的绝对值与对生活力影响的绝对值之间的相关性不强。对影响猪鬃数的数量性状基因座（QTL）进行了50个P元件插入标记的细胞遗传学定位。产生了2个P-元件诱导的粗毛类等位基因和5个外毛类等位基因。单P元件诱变是在遗传位点水平上鉴定QTL的有效方法。
SingleP-element mutagenesis was used to construct 1094 lines withP[lArB] inserts on all three major chromosomes in an isogenic background previously free ofPelements. The effects of insertions on bristle number and on viability were assessed by comparison to 392 control lines. The variance and effects ofP-element inserts on bristle number and viability were larger than those inferred from spontaneous mutations. The distributions of effects on bristle number were symmetrical and highly leptokurtic, such that a few inserts with large effects caused most of the increase in variance. The distribution of effects on viability were negatively skewed and platykurtic. On average, the effects ofP-element insertions on bristle number were partly recessive and on viability were completely recessive.P-element inserts with large effects on bristle number tended to have reduced viability, but the correlation between the absolute value of the effects on bristle number and on viability was not strong. FiftyP-element inserts tagging quantitative trait loci (QTLs) with large effects on bristle number were mapped cytogenetically. TwoP-element-inducedscabrousalleles and fiveextramacrochaetaealleles were generated. SingleP-element mutagenesis is a powerful method for identifylng QTLs at the level of genetic locus.