Peripheral CRF activates myenteric neurons in the proximal colon through CRF1 receptor in conscious rats
Peripheral CRF activates myenteric neurons in the proximal colon through CRF1 receptor in conscious rats
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DOI：
10.1152/ajpgi.00434.2001
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发表时间：
2002-05-01
影响因子：
4.5
通讯作者：
Taché, Y
中科院分区：
文献类型：
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作者：
Miampamba, M;Maillot, C;Taché, Y
外周注射促肾上腺皮质激素释放因子（CRF）通过CRF 1受体诱导大鼠近端结肠簇状峰丛放电活动。我们研究了腹膜内CRF对清醒大鼠近端结肠神经节肌间神经细胞活性的影响，采用结肠纵肌/肌间神经丛整体标本的Fos免疫组织化学方法。在溶剂预处理的大鼠中，每个神经节仅有少量Fos免疫反应（IR）细胞（1.2 +/- 0.6）。CRF（10 μ g/kg ip）诱导Fos表达，19.6 +/- 2.1个细胞/神经节。CRF 1/CRF 2拮抗剂astressin（33 μ g/kg ip）和选择性CRF 1拮抗剂CP-154，526（20 mg/kg sc）阻止了近端结肠中腹膜内CRF诱导的Fos表达（Fos-IR细胞/神经节的数量：分别为2.7 +/- 1.2和1.0 +/- 1.0），而阿托品（1 mg/kg sc）则无影响。Fos与蛋白质基因产物9.5的双标记揭示了被对囊泡乙酰胆碱转运蛋白免疫反应的曲张纤维包围的活化细胞的神经元身份。Fos免疫反应主要存在于胆碱乙酰转移酶-IR神经细胞体中，而不是在NADPH-黄递酶阳性细胞中。这些结果表明，外周CRF通过CRF 1受体激活近端结肠肌间胆碱能神经元。
Corticotropin-releasing factor (CRF) injected peripherally induces clustered spike-burst activity in the proximal colon through CRF1 receptors in rats. We investigated the effect of intraperitoneal CRF on proximal colon ganglionic myenteric cell activity in conscious rats using Fos immunohistochemistry on the colonic longitudinal muscle/myenteric plexus whole mount preparation. In vehicle-pretreated rats, there were only a few Fos immunoreactive (IR) cells per ganglion (1.2 +/- 0.6). CRF (10 mug/kg ip) induced Fos expression in 19.6 +/- 2.1 cells/ ganglion. The CRF1/CRF2 antagonist astressin (33 mug/kg ip) and the selective CRF1 antagonist CP-154,526 (20 mg/kg sc) prevented intraperitoneal CRF-induced Fos expression in the proximal colon (number of Fos-IR cells/ ganglion: 2.7 +/- 1.2 and 1.0 +/- 1.0, respectively), whereas atropine (1 mg/kg sc) had no effect. Double labeling of Fos with protein gene product 9.5 revealed the neuronal identity of activated cells that were encircled by varicose fibers immunoreactive to vesicular acetylcholine transporter. Fos immunoreactivity was mainly present in choline acetyltransferase-IR nerve cell bodies but not in the NADPH-diaphorase-positive cells. These results indicate that peripheral CRF activates myenteric cholinergic neurons in the proximal colon through CRF1 receptor.