Detection of exosomal tyrosine receptor kinase B as a potential biomarker in ovarian cancer
Detection of exosomal tyrosine receptor kinase B as a potential biomarker in ovarian cancer
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检测外泌体酪氨酸受体激酶 B 作为卵巢癌潜在生物标志物
DOI：
10.1002/jcb.27923
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发表时间：
2019-04-01
影响因子：
4
通讯作者：
Gao, Qinglei
中科院分区：
文献类型：
--
作者：
Li, Wenqian;Lu, Yunhui;Gao, Qinglei
卵巢癌（OC）是一种由于缺乏特异性生物标志物而在晚期诊断的致命疾病。酪氨酸受体激酶B（Trk B）与OC的进展有关，是一种有前景的OC诊断和预后的生物标志物。循环外泌体作为各种疾病的生物标志物的发现使我们探索OC中的外泌体TrkB。我们前期的研究证实TrkB在OC组织中表达增高。在本研究中，我们专注于外泌体TrkB在OC中的检测。首先采用连续离心法从三种不同OC细胞系的条件培养液、OC患者血清和异种移植小鼠血清中收集Exosomes。然后，我们通过透射电子显微镜、NanoSight分析和典型外泌体蛋白标志物的表达来鉴定外泌体。Western blot检测TrkB在exosomes中的存在，酶联免疫吸附试验检测TrkB的表达。在这项研究中，我们证明了外泌体可以来自OC细胞系、OC异种移植裸鼠的血清和临床患者。我们的研究表明，血清外泌体TrkB可能被认为是OC的微创生物标志物。
Ovarian cancer (OC) is a lethal disease diagnosed at advanced stages due to the lack of specific biomarkers. Tyrosine receptor kinase B (TrkB), which has recently been found to be related to OC progression, represents a promising potential biomarker for OC diagnosis and prognosis. The discovery of circulating exosomes as biomarkers for various diseases led us to explore exosomal TrkB in OC. Our previous study proved that the expression of TrkB was elevated in OC tissues. In this study, we focused on the detection of exosomal TrkB in OC. Exosomes were first gathered from three different OC cell lines’ conditioned medium, serum samples of patients with OC as well as xenograft mice serum by serial centrifugation method. Then, we identified exosomes by transmission electron microscopy, NanoSight analysis, and expression of typical exosomal protein markers. The existence of TrkB in exosomes was measured by Western blot analysis, and the expression was detected by enzyme‐linked immunosorbent assay. In this study, we demonstrated that exosomes could derive from OC cell lines, serum from OC xenograft nude mice, and clinical patients. Our study shows that serum exosomal TrkB may be considered a minimally invasive biomarker for OC.