The amino acid sequence of the fluorescein isothiocyanate reactive site of lamb and rat kidney Na+- and K+-dependent ATPase.
The amino acid sequence of the fluorescein isothiocyanate reactive site of lamb and rat kidney Na+- and K+-dependent ATPase.
复制标题
羔羊和大鼠肾脏 Na 和 K 依赖性 ATP 酶的异硫氰酸荧光素反应位点的氨基酸序列。
DOI：
10.1016/0006-291x(84)90605-3
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发表时间：
1984
影响因子：
3.1
通讯作者：
Lane,LK
中科院分区：
文献类型：
--
作者：
Kirley,TL;Wallick,ET;Lane,LK
用5′-异硫氰酸黄绿素分别标记羊和大鼠肾脏中哇巴因敏感和不敏感的（Na，K）-ATP酶。标记的酶用胰蛋白酶消化产生可溶性肽，通过高效液相色谱法纯化并在气相测序仪上测序。两种物种的标记肽序列均为His-Leu-Leu-Val-Met-Lys-Gly-Ala-Pro-Glu-Arg。因此，似乎荧光素5′-异硫氰酸酯反应位点的一级结构，因此推测ATP结合位点，在哇巴因敏感和哇巴因不敏感的（Na，K）-ATP酶中是完全保守的。
Fluorescein 5′-isothiocyanate has been used to label ouabain sensitive and insensitive (Na,K)-ATPases from lamb and rat kidney, respectively. The labeled enzymes were digested with trypsin to generate soluble peptides, which were purified by high performance liquid chromatography and sequenced on a gas phase sequenator. The sequence of the labeled peptide from both species is His-Leu-Leu-Val-Met-Lys-Gly-Ala-Pro-Glu-Arg. Thus, it appears that the primary structure of the fluorescein 5′-isothiocyanate reactive site, and therefore presumably the ATP binding site, is completely conserved in ouabain sensitive and ouabain insensitive (Na,K)-ATPases.