Unveiling the orientation and dynamics of enzymes in unstructured artificial compartments of metal–organic frameworks (MOFs)
Unveiling the orientation and dynamics of enzymes in unstructured artificial compartments of metal–organic frameworks (MOFs)
复制标题
揭示金属有机框架 (MOF) 非结构化人工隔室中酶的方向和动力学
DOI：
10.1039/d2nr06659a
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发表时间：
2023
期刊：
影响因子：
6.7
通讯作者：
Yang, Zhongyu
中科院分区：
文献类型：
--
作者：
Pan, Yanxiong;Li, Qiaobin;Liu, Wei;Armstrong, Zoe;MacRae, Austin;Feng, Li;McNeff, Charles;Zhao, Pinjing;Li, Hui;Yang, Zhongyu
将酶限制在形状良好的MOF隔室中是模拟酶的细胞环境并确定其中的酶结构-功能关系的有希望的方法。然而，在细胞拥挤的情况下，酶也可以被限制在接近酶的形状/轮廓的非结构化空间中。因此，为了更好地了解酶在其生理环境中的作用，有必要研究这些非结构化空间中的酶。然而，实际上，创建接近酶轮廓的区室并探测其中的酶结构信息是具有挑战性的。在这里，为了证明原理，我们通过共结晶将模型酶溶菌酶限制在MOF的晶体缺陷中，其中溶菌酶作为MOF晶体支架生长的核，从而产生接近溶菌酶轮廓的非结构化空间，并确定酶的相对取向和动力学。这一努力对于理解近天然环境中的酶和指导模拟自然界如何限制酶的生物催化剂的合理设计非常重要。
Confining enzymes in well-shaped MOF compartments is a promising approach to mimic the cellular environment of enzymes and determine enzyme structure–function relationship therein. Under the cellular crowding, however, enzymes can also be confined in unstructured spaces that are close to the shapes/outlines of the enzyme. Therefore, for a better understanding of enzymes in their physiological environments, it is necessary to study enzymes in these unstructured spaces. However, practically it is challenging to create compartments that are close to the outline of an enzyme and probe enzyme structural information therein. Here, for proof-of-principle, we confined a model enzyme, lysozyme, in the crystal defects of a MOF via co-crystallization, where lysozyme served as the nuclei for MOF crystal scaffolds to grow on so that unstructured spaces close to the outline of lysozyme are created, and determined enzyme relative orientation and dynamics. This effort is important for understanding enzymes in near-native environments and guiding the rational design of biocatalysts that mimic how nature confines enzymes.