Miniaturized probe for femtosecond laser microsurgery and two-photon imaging
Miniaturized probe for femtosecond laser microsurgery and two-photon imaging
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DOI：
10.1364/oe.16.009996
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发表时间：
2008-06-23
期刊：
影响因子：
3.8
通讯作者：
Ben-Yakar, Adela
中科院分区：
文献类型：
--
作者：
Hoy, Christopher L.;Durr, Nicholas J.;Ben-Yakar, Adela
结合双光子荧光显微镜和飞秒激光显微手术作为一个强大的“寻找和治疗”的工具，有许多潜在的生物医学应用。为了开发这样一个工具，我们展示了一个小型化的探头，它结合了这些技术在一个紧凑的外壳。该设备的尺寸为10 x 15 x 40 mm（3），使用空芯光子晶体光纤以80 MHz重复率提供飞秒激光脉冲用于成像，1 kHz用于显微手术。一种快速双轴微机电系统扫描镜在共振驱动下产生每秒10帧的李萨如光束扫描。视场直径为310 μ m，横向和轴向分辨率分别为1.64 μ m和16.4 μ m。结合成像和显微手术证明使用活癌细胞。(c)2008年美国光学学会。
Combined two-photon fluorescence microscopy and femtosecond laser microsurgery has many potential biomedical applications as a powerful "seek-and-treat" tool. Towards developing such a tool, we demonstrate a miniaturized probe which combines these techniques in a compact housing. The device is 10 x 15 x 40 mm(3) in size and uses an air-core photonic crystal fiber to deliver femtosecond laser pulses at 80 MHz repetition rate for imaging and 1 kHz for microsurgery. A fast two-axis microelectromechanical system scanning mirror is driven at resonance to produce Lissajous beam scanning at 10 frames per second. Field of view is 310 mu m in diameter and the lateral and axial resolutions are 1.64 mu m and 16.4 mu m, respectively. Combined imaging and microsurgery is demonstrated using live cancer cells. (c) 2008 Optical Society of America.