Photodynamic triggering of calcium oscillation in the isolated rat pancreatic acini
Photodynamic triggering of calcium oscillation in the isolated rat pancreatic acini
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DOI：
10.1111/j.1469-7793.1997.047bf.x
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发表时间：
1997-10-01
影响因子：
5.5
通讯作者：
Kanno, T
中科院分区：
文献类型：
--
作者：
Cui, ZJ;Kanno, T
1. Photodynamic agents, due to their photon-dependent selective activation, can selectively activate a number of physiological processes and may directly modulate signal transduction in a number of cells including pancreatic acinar cells.2. Activation of the photodynamic agent sulphonated aluminium phthalocyanine (SALPC) triggered recurrent cytosolic calcium ([Ca2+](i)) spiking in pancreatic acinar cells.3. The photodynamically triggered calcium spiking could be blocked by phosphatidylinositol-specific phospholipase C (PI-PLC) inhibitor U73122, but not by phosphatidylcholine-specific phospholipase C inhibitor D809.4. Removal of extracellular Ca2+ abolished spiking, as did 2-aminoethoxydiphenylborate (2-APB), an inhibitory modulator of IP3-mediated Ca2+ release from intracellular stores.5. These data suggest that SALPC photodynamic action may permanently fix PI-PLC in an active conformation, and this produced recurrent [Ca2+](i) spiking.
