Control of a Nanoscopic-to-Macroscopic Transition: Modulated Phases in Four-Component DSPC/DOPC/POPC/Chol Giant Unilamellar Vesicles
Control of a Nanoscopic-to-Macroscopic Transition: Modulated Phases in Four-Component DSPC/DOPC/POPC/Chol Giant Unilamellar Vesicles
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DOI：
10.1016/j.bpj.2011.06.019
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发表时间：
2011-07-20
影响因子：
3.4
通讯作者：
Feigenson, Gerald W.
中科院分区：
文献类型：
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作者：
Konyakhina, Tatyana M.;Goh, Shih Lin;Feigenson, Gerald W.
我们发现在四组分脂质双层混合物 DSPC/DOPC/POPC/Chol 的液体有序 + 液体无序共存区域内的特定组成区域中存在调制相形态。通过控制脂质成分，我们可以看到不同类型的调节液-液相形态，包括巨型单层囊泡中的线性、不规则和有角特征。我们结合使用了共焦、双光子、宽视场荧光和微分干涉显微镜，并使用严格的控制来最大限度地减少光引起的伪影。这些研究表明，膜筏的尺寸和形态可以通过胆固醇和高熔点脂质混合物中低熔点脂质的浓度和类型来控制。
We have found modulated phase morphology in a particular region of composition within the liquid-ordered + liquid-disordered coexistence region in the four-component lipid bilayer mixture DSPC/DOPC/POPC/Chol. By controlling lipid composition, we could see distinct types of modulated liquid-liquid phase morphologies, including linear, irregular, and angular features in giant unilamellar vesicles. We used a combination of confocal, two-photon, wide-field fluorescence, and differential interference contrast microscopies, and used stringent controls to minimize light-induced artifacts. These studies establish that both the size and morphology of membrane rafts can be controlled by the concentration and the type of low-melting lipid in mixtures with cholesterol and a high-melting lipid.