Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection
Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection
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DOI：
10.1016/j.trim.2015.05.002
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发表时间：
2015-09-01
影响因子：
1.5
通讯作者：
Amirzargar, Aliakbar
中科院分区：
文献类型：
--
作者：
Soltaninejad, Ehsan;Nicknam, Mohammad Hossein;Amirzargar, Aliakbar
背景：MicroRNA (miRNA) 调节大部分编码基因和蛋白质。在本研究中，我们旨在研究与正常同种异体移植物（NA）相比，患有急性 T 细胞介导的排斥反应（ATCMR）的同种异体肾移植受者的配对活检和外周血单核细胞（PBMC）样本中 miR-142-5p、miR-142-3p、miR-155 和 miR-223 的表达水平。 17 名 ATCMR 受者和 18 名 NA 受者的 PBMC 样本。 结果：我们的结果表明，所有研究的 miRNA 的移植物内表达水平在 ATCMR 中均显着高于 NA。然而，对于 PBMC 样本，ATCMR 中的 miR-142-3p 和 miR-223 比 NA 中显着增加。接受者操作特征（ROC）分析显示，活检样本中的miR-142-5p、miR-142-3p、miR-155和miR-223以及PBMC样本中的miR-142-3p和miR-223可以区分ATCMR和NA受者。结论：据报道，移植物内高表达的miRNA在ATCMR过程的发病机制中具有深远的作用。我们的结果表明，活检中所有研究的 miRNA 以及 PBMC 样本中 miR-142-3p 和 miR-223 的高表达可用作区分 ATCMR 患者与 NA 的提示性诊断工具。 (C) 2015 Elsevier B.V. 保留所有权利。
Background: MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA).Methods: In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA.Results: Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients.Conclusion: It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA. (C) 2015 Elsevier B.V. All rights reserved.