Subangstrom single-molecule measurements of motor proteins using a nanopore.
Subangstrom single-molecule measurements of motor proteins using a nanopore.
复制标题
DOI：
10.1038/nbt.3357
复制
发表时间：
2015-10
影响因子：
46.9
通讯作者：
Gundlach JH
中科院分区：
文献类型：
--
作者：
Derrington IM;Craig JM;Stava E;Laszlo AH;Ross BC;Brinkerhoff H;Nova IC;Doering K;Tickman BI;Ronaghi M;Mandell JG;Gunderson KL;Gundlach JH
Present techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ~300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ~40 picometer sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.
