Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (Publication with Expression of Concern)
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (Publication with Expression of Concern)
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DOI：
10.2807/1560-7917.es.2020.25.3.2000045
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发表时间：
2020-01-23
期刊：
影响因子：
19
通讯作者：
Drosten, Christian
中科院分区：
文献类型：
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作者：
Corman, Victor M.;Landt, Olfert;Drosten, Christian
背景资料：最近出现的新型冠状病毒（2019-nCoV）的持续爆发对公共卫生实验室构成了挑战，因为病毒分离物不可用，而越来越多的证据表明，疫情比最初想象的更广泛，并且通过旅行者进行的国际传播确实已经发生。目的：我们的目标是开发和部署强大的诊断方法，用于公共卫生实验室环境，而无需病毒材料。研究方法：在这里，我们提出了一个经过验证的2019-nCoV诊断工作流程，其设计依赖于2019-nCoV与SARS冠状病毒的密切遗传相关性，利用合成核酸技术。结果：该工作流程可靠地检测到2019-nCoV，并进一步区分2019-nCoV与SARS-CoV。通过学术和公共实验室之间的协调，我们基于297份含有全谱人类呼吸道病毒的原始临床标本确认了检测试剂盒的排他性。通过欧洲联盟的一个基础设施项目-欧洲病毒档案-全球（EVAg）提供控制材料。结论：本研究表明，通过国家和欧洲研究网络中的学术和公共实验室的协调，取得了巨大的反应能力。
Background: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. Aim: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. Methods: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. Results: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive - Global ( EVAg), a European Union infrastructure project. Conclusion: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.