Flow cytometry as a new method to quantify the cellular content of human saliva and its relation to gingivitis
Flow cytometry as a new method to quantify the cellular content of human saliva and its relation to gingivitis
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DOI：
10.1016/s0009-8981(02)00062-1
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发表时间：
2002-07-01
影响因子：
5
通讯作者：
Martens, LC
中科院分区：
文献类型：
--
作者：
Aps, JKM;Van den Maagdenberg, K;Martens, LC
背景：用常规显微镜小室计数的方法测定唾液中的细胞含量是一项非常耗时且对操作员敏感的程序。本研究主要利用流式细胞仪检测唾液中的细胞含量。对红细胞、白细胞、上皮细胞和细菌进行了量化，并将结果与龋齿经历和牙周炎的存在进行了比较。方法：采用UF-100(R)流式细胞仪对258例未经离心的石蜡刺激唾液标本进行分析。建立唾液红细胞、白细胞、上皮细胞和细菌计数参考值。记录患龋率(DMF)和牙周炎情况。结果：流式细胞术不能评价患者的龋病经历或患龋率。然而，唾液流式细胞术可能有助于确定个体患牙龈炎的风险：在患牙周炎的个体中观察到唾液白细胞显著增加。以10(3)个白细胞-1唾液为界值，灵敏度为76%，特异度为45%。其他分析结果在患牙周炎和不患牙周炎的个体之间没有显著差异。结论：石蜡刺激细胞的流式细胞术分析。人类唾液似乎是一种很有前途的诊断或预测工具，未来应该对口咽部疾病进行进一步的研究，如扁桃体炎和牙周炎。(C)2002年，爱思唯尔科学公司出版。
Background: Determining the cellular content of saliva by means of conventional microscopy chamber counting is a very time-consuming and operator-sensitive procedure. This study concentrated on the use of flow cytometry to examine the cellular content of saliva. Erythrocytes, leukocytes, epithelial cells and bacteria were quantified and the results were compared with caries experience and the presence of gingivitis. Methods: 258 uncentrifuged vortexed paraffin-stimulated saliva samples (112 males and 146 females) were analyzed with the UF-100(R) flow cytometer. Salivary reference values were established for erythrocyte, leukocyte, epithelial cell and bacterial Count. Caries experience (DMF) and the presence of gingivitis were recorded. Results: Caries experience or caries risk could not be assessed with flow cytometry. However, salivary flow cytometry may be useful in determining an individual's risk for gingivitis: a significant increase in salivary leukocytes was observed in individuals with gingivitis. At a cut-off level of 10(3) leukocytes mul(-1) saliva, a sensitivity of 76% and a specificity of 45% was obtained. Other analytes were not significantly different between individuals with and without gingivitis. Conclusion: Flow cytometry of paraffin-stimulated. human saliva seems a promising diagnostic or predictive tool and further investigations of diseases of the oro-pharyngeal loge, such as tonsillitis and periodontitis, should be carried out in the future. (C) 2002 Published by Elsevier Science B.V.