Effects of Benzo(a)pyrene on the Contractile Function of the Thoracic Aorta of Sprague-dawley Rats
Effects of Benzo(a)pyrene on the Contractile Function of the Thoracic Aorta of Sprague-dawley Rats
复制标题
苯并(a)芘对大鼠胸主动脉收缩功能的影响
DOI：
10.3967/0895-3988.2012.05.008
复制
发表时间：
2012-10-01
影响因子：
3.5
通讯作者：
Yang Jun
中科院分区：
文献类型：
--
作者：
Gan Tie Er;Xiao Su Ping;Yang Jun
Objective To evaluate the possible vascular effects of an environment carcinogen benzo(a)pyrene (BaP).Methods The cytotoxicit of BaP and rat liver 59 (0.25 mg/mL)-activated BaP were examined by MU assay. Thoracic aortic rings were dissected from Sprague-Dawley rats. Contraction of aortic rings was induced by 60 mmol/L KCl or 10(-6) mol/L phenylephrine (PE) in an ex-vivo perfusion system after BaP (100 mu mol/L) incubation for 6 h. [Ca2+](i) was measured using Fluo-4/AM. For in-vivo treatment, rats were injected with BaP for 4 weeks (10 mg/kg, weekly, i.p.).Results BaP (1-500 mu m) did not significantly affect cell viability; S9-activated BaP stimulated cell proliferation. BaP did not affect the contractile function of endothelium-intact or -denuded aortic rings. BaP did not affect ATP-induced ([Ca2+](i)) increases in human umbilical vein endothelial cells. In BaP-treated rats, heart rate and the number of circulating inflammatory cells were not affected. Body weight decreased while blood pressure increased significantly. The maximum aortic contractile responses to PE and KCl and the maximum aortic relaxation response to acetylcholine were significantly decreased by 25.0%, 34.2%, and 10.4%, respectively.Conclusion These results suggest, in accordance with its DNA-damaging properties, that metabolic activation is a prerequisite for BaP-induced cardiovascular toxicity.
