Linkage between the leader sequence and leader RNA production in Borna disease virus-infected cells.
Linkage between the leader sequence and leader RNA production in Borna disease virus-infected cells.
复制标题
博尔纳病病毒感染细胞中前导序列和前导 RNA 产生之间的联系。
DOI：
10.1016/j.virol.2017.07.011
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Tomonaga K.
中科院分区：
文献类型：
--
作者：
Honda T;Sofuku K;Kojima S;Yamamoto Y;Ohtaki N;Tomonaga K.
非节段负链RNA（non-segmented，negative-strand RNA，NNS）病毒基因组的3′-非翻译区（3′-untranslated region，UTR）被称为前导序列（leader sequence），是病毒复制和转录的启动子。NNS RNA病毒也使用该序列作为模板来合成具有未知功能的前导RNA（leRNA）。博尔纳病病毒（BDV）是独特的，因为它建立了一个持续的感染和复制的细胞核。尚未有报道证明在BDV感染期间存在leRNA。在这里，我们报告BDV从基因组的3′-UTR合成leRNA。它们起始于3′-UTR的第5位，终止于核蛋白基因的转录起始信号。leRNA的产生水平与病毒复制和转录水平无关。另一方面，3′-UTR的突变影响leRNA的产生。我们的研究结果为BDV的生命周期增加了一种新的病毒转录物，并阐明了BDV的复制和/或转录。
The 3′-untranslated region (UTR) of the non-segmented, negative-strand (NNS) RNA viral genome is called the leader sequence, and functions as the promoter for viral replication and transcription. NNS RNA viruses also use the sequence as a template to synthesize leader RNAs (leRNAs) with unknown functions. Borna disease virus (BDV) is unique because it establishes a persistent infection and replicates in the nucleus. No report has yet demonstrated the presence of leRNAs during BDV infection. Here, we report that BDV synthesizes leRNAs from the 3′-UTR of the genome. They started at position 5 in the 3′-UTR and ended by the transcription start signal of the nucleoprotein gene. The level of leRNA production is not correlated with the levels of viral replication and transcription. On the other hand, mutation of the 3′-UTR affects leRNA production. Our findings add a novel viral transcript to the BDV life cycle and shed light on BDV replication and/or transcription.