Dopaminergic amacrine cells express opioid receptors in the mouse retina.
Dopaminergic amacrine cells express opioid receptors in the mouse retina.
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DOI：
10.1017/s0952523812000156
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发表时间：
2012-05
影响因子：
1.9
通讯作者：
Vigh, Jozsef
中科院分区：
文献类型：
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作者：
Gallagher, Shannon K.;Anglen, Julia N.;Mower, Justin M.;Vigh, Jozsef
阿片受体的存在已被证实的各种技术在脊椎动物视网膜，包括那些哺乳动物;然而，在大多数报告中，这些受体的位置已被限制在视网膜区域，而不是特定的细胞类型。同时，我们对视网膜中阿片信号生理功能的了解仅基于少数研究。迄今为止，最好的记录阿片类药物的作用是调节视网膜多巴胺的释放，这已被证明在各种脊椎动物物种。尽管如此，目前尚不清楚阿片类药物是否可以通过DAC表达的阿片受体直接影响多巴胺能无长突细胞（DAC）。本研究使用免疫组织化学方法，试图确定（1）μ-和δ-阿片受体（分别为MORs和DORs）是否存在于小鼠视网膜中，如果存在，（2）它们是否由DAC表达。我们发现，莫尔和DOR免疫标记与内层视网膜中的多种细胞类型相关，这表明阿片类药物可能会影响哺乳动物视网膜回路中多个部位的视觉信息处理。具体而言，用DAC分子标志物抗酪氨酸羟化酶抗体进行的共标记研究表明，莫尔和DOR免疫标记均定位于DAC。这些发现预测阿片类药物可以通过莫尔和DOR信号直接影响小鼠视网膜中的DAC，并且可能调节多巴胺释放，如在其他哺乳动物和非哺乳动物视网膜中所报道的。
The presence of opioid receptors has been confirmed by a variety of techniques in vertebrate retinas including those of mammals; however, in most reports the location of these receptors has been limited to retinal regions rather than specific cell-types. Concurrently, our knowledge of the physiological functions of opioid signaling in the retina is based on only a handful of studies. To date, the best documented opioid effect is the modulation of retinal dopamine release, which has been shown in a variety of vertebrate species. Nonetheless, it is not known if opioids can affect dopaminergic amacrine cells (DACs) directly, via opioid receptors expressed by DACs. This study, using immunohistochemical methods, sought to determine whether (1) μ- and δ-opioid receptors (MORs and DORs, respectively) are present in the mouse retina, and if present, (2) are they expressed by DACs. We found that MOR and DOR immunolabeling was associated with multiple cell-types in the inner retina, suggesting that opioids might influence visual information processing at multiple sites within the mammalian retinal circuitry. Specifically, colabeling studies with the DAC molecular marker anti-tyrosine hydroxylase antibody showed that both MOR and DOR immunolabeling localize to DACs. These findings predict that opioids can affect DACs in the mouse retina directly, via MOR and DOR signaling, and might modulate dopamine release as reported in other mammalian and non-mammalian retinas.