Effects of a probiotic strain of Enterococcus faecium on the rate of natural chlamydia infection in swine
Effects of a probiotic strain of Enterococcus faecium on the rate of natural chlamydia infection in swine
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DOI：
10.1128/iai.73.7.4346-4353.2005
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发表时间：
2005-07-01
影响因子：
3.1
通讯作者：
Wieler, LH
中科院分区：
文献类型：
--
作者：
Pollmann, M;Nordhoff, M;Wieler, LH
衣原体是专性细胞内病原体，其引起与家畜和人类的广泛疾病相关的感染。此外，大部分动物可能成为持续感染的无症状携带者，并成为其他动物的宿主，这些动物也会传播这些潜在的人畜共患病病原体。因此，减少动物的衣原体负荷是非常重要的，并且由于大规模的抗生素治疗既不期望也不可行，因此需要替代的预防手段。在这里，我们进行了一项研究，比较了屎肠球菌益生菌菌株在降低猪自然感染率和衣原体脱落方面的功效。衣原体科的存在下，通过种特异性PCR的母猪粪便样品，在仔猪出生前三个时间进行检测。还检查了对照组或益生菌组衣原体阳性母猪分娩的仔猪在不同年龄的衣原体感染频率。对照组中85%的仔猪被发现为衣原体阳性，而益生菌组中仅60%的仔猪被发现衣原体，荧光原位杂交和免疫组织学结果证实了这一点，表明对照组的感染率较高。除了益生菌组中衣原体阳性仔猪的频率降低外，阳性样品出现的时间也延迟了。据我们所知，这些数据首次表明，益生菌菌株的E。粪便可以降低仔猪由专性细胞内病原体引起的携带感染率。
Chlamydiae are obligately intracellular pathogens which cause infections associated with a broad range of diseases in both livestock and humans. In addition, a large proportion of animals may become persistently infected asymptomatic carriers and serve as reservoirs for other animals which also shed these potential zoonotic pathogens. Reducing the chlarnydial load of animals is therefore of major importance, and since large-scale antibiotic treatment is neither desired nor feasible, alternative means of prevention are needed. Here we performed a study comparing the efficacy of a probiotic strain of Enterococcus faecium on the reduction of both the rate of natural infection and the shedding of chlamydiae in swine. The presence of Chlamydiaceae was detected by species-specific PCR of fecal samples of sows taken at three times prior to the birth of piglets. Piglets delivered from chlamydia-positive sows in either the control or the probiotic group were also examined for the frequency of chlamydiae at various ages. Eighty-five percent of the piglets from the control group were found to be chlamydia positive, whereas chlamydiae were found in only 60% of piglets from the probiotic group, results confirmed by fluorescence in situ hybridization and immunohistology, which showed higher rates of infection in the control group. In addition to the reduced frequency of chlamydia-positive piglets in the probiotic group, the time of appearance of positive samples was delayed. To our knowledge, these data show for the first time that a probiotic strain of E. faecium can reduce the rate of carryover infections of piglets by obligate intracellular pathogens.