Directed evolution: Creating biocatalysts for the future
Directed evolution: Creating biocatalysts for the future
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DOI：
10.1016/s0009-2509(96)00288-6
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发表时间：
1996-12-01
影响因子：
4.7
通讯作者：
Arnold, FH
中科院分区：
文献类型：
--
作者：
Arnold, FH
设计新酶的一种有效方法是指导它们的体外进化。通过在静止管中模拟达尔文进化的关键过程，可以探索酶的功能，而不受生命系统内功能的限制。通过多代随机突变和重组以及筛选改进的变体，已经开发出有效的策略来设计新的酶。我们对工业上重要的生物催化剂的研究结果强调了这种“进化”方法在蛋白质工程中的优势。(摘自1995年9月21日在第一届国家工程院“工程前沿”研讨会上的一次演讲。)版权所有(C)1996爱思唯尔科学有限公司
An effective approach to engineering new enzymes is to direct their evolution in vitro. By mimicking key processes of Darwinian evolution in the rest tube, the functions of enzymes can be explored free from the constraints of function within a living system. Efficient strategies for engineering new enzymes by multiple generations of random mutagenesis and recombination coupled with screening for improved variants have been developed. Our results with industrially important biocatalysts underscore the advantages of this 'evolutionary' approach to protein engineering. (From a talk presented at the first National Academy of Engineering ''Frontiers of Engineering'' Symposium, 21 September 1995.) Copyright (C) 1996 Elsevier Science Ltd