Atropine dissociates complexes of muscarinic acetylcholine receptor and guanine nucleotide-binding protein in heart membranes.
Atropine dissociates complexes of muscarinic acetylcholine receptor and guanine nucleotide-binding protein in heart membranes.
复制标题
阿托品解离心膜中毒蕈碱乙酰胆碱受体和鸟嘌呤核苷酸结合蛋白的复合物。
DOI：
10.1016/0014-5793(91)80680-2
复制
发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
Luthin,GR
中科院分区：
文献类型：
--
作者：
Matesic,DF;Luthin,GR
毒蕈碱乙酰胆碱受体和鸟嘌呤核苷酸结合蛋白（G蛋白）的复合物在激动剂卡巴胆碱存在下形成。复合物在去除激动剂后保持稳定，并在随后的心脏膜溶解和纯化中存活。当在除去激动剂后加入拮抗剂阿托品时，受体从G蛋白上解离。这是第一次直接证明受体-G蛋白复合物通过拮抗剂的结合而不稳定。
Complexes of muscarinic acetylcholine receptor and guanine nucleotide‐binding protein (G protein) are formed in the presence of the agonist carbachol. The complexes remain stable after removal of agonist, and survive subsequent solubilization from cardiac membranes and purification. Dissociation of the receptor from the G protein occurs when the antagonist atropine is added following removal of agonist. This is the first direct demonstration of destabilization of receptor—G protein complexes by the binding of an antagonist.