De novo DNA methyltransferase DNMT3A: Regulation of oligomeric state and mechanism of action in response to pH changes
De novo DNA methyltransferase DNMT3A: Regulation of oligomeric state and mechanism of action in response to pH changes
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DOI：
10.1016/j.bbagen.2015.02.003
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发表时间：
2015-06-01
影响因子：
3
通讯作者：
Reich, Norbert O.
中科院分区：
文献类型：
--
作者：
Holz-Schietinger, Celeste;Reich, Norbert O.
背景资料：人DNMT3A的寡聚状态在功能上是重要的，并且已知癌细胞经历pH（细胞内）的变化。此外，甲基化和持续合成能力assessment.Conclusions：生理相关的pH值的变化导致DNMT3A寡聚体组成的变化，这对DNMT3A function.General意义有显着的后果：发生在癌细胞内的pH值的变化改变了DNMT3A的寡聚体状态和功能，这可能有助于在体内观察到的基因组DNA甲基化的变化。由爱思唯尔公司出版
Background: The oligomeric state of the human DNMT3A is functionally important and cancer cells are known to undergo changes in pH (intracellular).Methods: Light scattering, gel filtration, and fluorescence anisotropy. Also, methylation and processivity assays.Conclusions: Physiologically relevant changes in pH result in changes in DNMT3A oligomer composition which have dramatic consequences on DNMT3A function.General significance: The pH changes which occur within cancer cells alter the oligomeric state and function of DNMT3A which could contribute to changes in genomic DNA methylation observed in vivo. Published by Elsevier B.V.