Testosterone induces a rapid stimulation of endocytosis, amino acid and hexose transport in mouse kidney cortex.
Testosterone induces a rapid stimulation of endocytosis, amino acid and hexose transport in mouse kidney cortex.
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DOI：
10.1016/0006-291x(82)91116-0
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发表时间：
1982-05
影响因子：
3.1
通讯作者：
H. Koenig;A. Goldstone;C. Lu
中科院分区：
文献类型：
--
作者：
H. Koenig;A. Goldstone;C. Lu
睾酮诱导快速（< 1 分钟）刺激内吞作用、氨基酸和己糖转运，通过小鼠肾皮质切片中 HRP、 14 C-AIB 和 3 H-DG 的温度敏感摄取来测量。摄取的激素增量持续至少 60-120 分钟，表现出时间、能量和 Na+ 依赖性，并随底物和睾酮浓度而变化。睾酮在 10− 8 至 10− 7 M 时最有效。过氧化物酶组织化学表明 HRP 摄取的激素增加仅限于近端小管。睾酮比双氢睾酮更有效，而醋酸环丙孕酮、雄酮和地塞米松对这种吸收几乎没有或没有刺激作用。雄激素不敏感的 tfm Y 小鼠的肾脏切片对睾酮没有反应。内吞作用、氨基酸和己糖转运的快速增加可能代表靶细胞表面膜对睾酮的直接、受体介导的反应。
Testosterone induced a rapid (< 1 min) stimulation of endocytosis, amino acid and hexose transport, measured by the temperature-sensitive uptake of HRP, 14 C-AIB and 3 H-DG, in mouse kidney cortex slices. The hormonal increment in uptake persisted for at least 60–120 min, showed time-, energy-, and Na+-dependence, and varied with substrate and testosterone concentration. Testosterone was maximally effective at 10− 8 to 10− 7 M. Peroxidase histochemistry indicated that the hormonal increase in HRP uptake is restricted to proximal tubules. Testosterone was more effective than DHT, whereas cyproterone acetate, androsterone and dexamethasone had little or no stimulating effect on this uptake. Kidney slices from androgen-insensitive tfm Y mice did not respond to testosterone. The rapid increase in endocytosis, amino acid and hexose transport may represent a direct, receptor-mediated response of the surface membrane of target cells to testosterone.