Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink.
Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink.
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DOI：
10.1038/ncomms4935
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发表时间：
2014-06-02
影响因子：
16.6
通讯作者：
Cho DW
中科院分区：
文献类型：
--
作者：
Pati F;Jang J;Ha DH;Won Kim S;Rhie JW;Shim JH;Kim DH;Cho DW
能够在三个维度上打印构成组织的所有组件（细胞和基质材料）并形成图案，以生成类似于组织的结构，这是生物打印令人兴奋的前景。然而，迄今为止用于生物打印的大多数基质材料不能代表天然细胞外基质（ECM）的复杂性，因此无法重建内在的细胞形态和功能。在这里，我们开发了一种使用新型脱细胞细胞外基质（dECM）生物墨水对载有细胞的构建体进行生物打印的方法，该生物墨水能够提供有利于三维结构组织生长的优化微环境。我们展示了使用组织特异性 dECM 生物墨水（包括脂肪、软骨和心脏组织）开发的生物打印过程的多功能性和灵活性，能够为细胞植入、存活和长期功能提供关键线索。我们使用生物打印方法实现了打印的 dECM 结构的高细胞活力和功能性。 3D 打印在生物材料上的应用为组织工程带来了有趣的可能性。在这里，作者表明，源自细胞外基质的打印介质可以应用于打印具有增强的细胞相容性的组织类似物。
The ability to print and pattern all the components that make up a tissue (cells and matrix materials) in three dimensions to generate structures similar to tissues is an exciting prospect of bioprinting. However, the majority of the matrix materials used so far for bioprinting cannot represent the complexity of natural extracellular matrix (ECM) and thus are unable to reconstitute the intrinsic cellular morphologies and functions. Here, we develop a method for the bioprinting of cell-laden constructs with novel decellularized extracellular matrix (dECM) bioink capable of providing an optimized microenvironment conducive to the growth of three-dimensional structured tissue. We show the versatility and flexibility of the developed bioprinting process using tissue-specific dECM bioinks, including adipose, cartilage and heart tissues, capable of providing crucial cues for cells engraftment, survival and long-term function. We achieve high cell viability and functionality of the printed dECM structures using our bioprinting method. The application of 3D printing to biomaterials presents interesting possibilities for tissue engineering. Here, the authors show that a printing medium derived from an extracellular matrix can be applied to printing tissue analogues with enhanced cell compatibility.