TEMPORAL AND SPATIAL PATTERNS OF TRANSFORMING GROWTH FACTOR-BETA-1 EXPRESSION IN DEVELOPING RAT MOLARS
TEMPORAL AND SPATIAL PATTERNS OF TRANSFORMING GROWTH FACTOR-BETA-1 EXPRESSION IN DEVELOPING RAT MOLARS
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DOI：
10.1016/0003-9969(90)90015-3
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发表时间：
1990-01-01
影响因子：
3
通讯作者：
BUTLER, WT
中科院分区：
文献类型：
--
作者：
DSOUZA, RN;HAPPONEN, RP;BUTLER, WT
TGF-β家族的调节肽影响胚胎发育的各个方面。最近的免疫定位和原位杂交研究表明，特定的时间和组织依赖性表达的TGF-β 1在发育中的小鼠胚胎。本研究的目的是评估TGF-β-1在大鼠磨牙中的分布在不同的发展阶段，使用一个良好的特点，高度特异性的TGF-β-1抗体，免疫组织化学检测方法。TGF-β-1强烈免疫定位于钟状发育阶段的外胚层来源的星状网状组织和牙乳头间充质内。在成釉细胞的分化和分泌阶段，乳头层和减少的牙器官中也有明显的免疫染色。在冠状组织的形成过程中，在萌出前期，免疫反应性TGF-β 1明显定位于覆盖牙胚的牙囊内。TGF-β 1表达的时空模式似乎与牙齿发育过程中形态发生、组织发生和细胞分化中的特定事件相关。
Regulatory peptides of the TGF-beta family affect various aspects of embryonic development. Recent immunolocalization and in situ hybridization studies have demonstrated a specific time- and tissue-dependent expression of TGF-beta-1 in the developing mouse embryo. The purpose of this study was to evaluate the distribution of TGF-beta-1 within rat molars at different stages of development, using a well-characterized antibody, highly specific for TGF-beta-1, and immunohistochemical methods of detection. TGF-beta-1 was immunolocalized intensely within the ectodermally derived stellate reticulum and the mesenchyme of the dental papilla at the bell stage of development. Marked immunostaining was also evident in the papillary layer and the reduced dental organ subjacent to ameloblasts in the differentiation and secretory phases of amelogenesis. During the formation of coronal tissues and in the pre-eruptive phase, immunoreactive TGF-beta-1 was localized conspicuously within the dental follicle overlying the tooth germ. This temporospatial pattern of expression of TGF-beta-1 appears to correlate with specific events in morphogenesis, histogenesis and cytodifferentiation during tooth development.