Comparative characterization of hair follicle dermal stem cells and bone marrow mesenchymal stem cells
Comparative characterization of hair follicle dermal stem cells and bone marrow mesenchymal stem cells
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DOI：
10.1089/scd.2006.15.49
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发表时间：
2006-02-01
影响因子：
4
通讯作者：
Genever, PG
中科院分区：
文献类型：
--
作者：
Hoogduijn, MJ;Gorjup, E;Genever, PG
比较毛囊源性真皮干细胞和骨髓间充质干细胞的生长和分化特性。从Wistar大鼠胡须中分离毛囊真皮细胞，从相同动物的股骨中分离骨髓MSC。贴壁毛囊真皮细胞在含血清培养基中呈成纤维细胞形态，为CD 44（+）、CD 7（+）、CD 90（+）和CD 34（+），群体倍增时间为27 h。从骨髓中分离的MSC表现出相似的形态和群体倍增时间，并表达相同的细胞表面标志物。暴露于适当的诱导刺激后，两种细胞群都有能力分化成各种间充质谱系，如成骨细胞、脂肪细胞、软骨细胞和肌细胞，并表达神经祖细胞标志物。毛囊和骨髓来源的细胞的分化率和程度非常相似，而毛囊间真皮细胞未能分化。我们在毛囊真皮干细胞和骨髓间充质干细胞中检测到端粒酶活性，并证明它们具有克隆扩增的能力。在离体分析中，我们确定了假定的真皮干细胞在毛囊的真皮鞘和真皮乳头中的存在。因此，毛囊可能是一个合适的，可获得的MSC来源。
We compared the growth and differentiation characteristics of hair follicle-derived dermal stem cells with bone marrow mesenchymal stem cells (MSCs). Follicular dermal cells were isolated from whisker hairs of Wistar rats and bone marrow MSCs were isolated from femora of the same animals. The adherent hair follicle dermal cells showed a fibroblastic morphology in serum-containing culture medium, were CD44(+), CD7(+), CD90(+), and CD34(+), and had a population doubling time of 27 h. MSCs isolated from the bone marrow showed a similar morphology and population doubling time and expressed the same cell-surface markers. Following exposure to appropriate induction stimuli, both cell populations had the capacity to differentiate into various mesenchymal lineages, such as osteoblasts, adipocytes, chondrocytes, and myocytes and expressed neuroprogenitor cell markers. The rate and extent of differentiation were remarkably similar for both hair follicle- and bone marrow-derived cells, whereas interfollicular dermal cells failed to differentiate. We identified telomerase activity in follicle dermal stem cells and marrow MSCs and demonstrated that they were capable of clonal expansion. In ex vivo analyses, we identified the presence of putative dermal stem cells in the dermal sheath and dermal papillae of the hair follicle. Consequently, the hair follicle may represent a suitable, accessible source for MSCs.