Acceleration of partial-thickness burn wound healing with topical application of heparin-binding EGF-like growth factor (HB-EGF)
Acceleration of partial-thickness burn wound healing with topical application of heparin-binding EGF-like growth factor (HB-EGF)
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DOI：
10.1097/00004630-199803000-00002
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发表时间：
1998-03-01
期刊：
影响因子：
--
通讯作者：
Besner, GE
中科院分区：
文献类型：
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作者：
Cribbs, RK;Luquette, MH;Besner, GE
肝素结合的EGF样生长因子已经在人的烧伤创面液体和切除的人部分深度烧伤的上皮细胞中被发现。在本研究中，通过将纯化的重组肝素结合的EGF样生长因子掺入局部应用于小鼠部分深度烧伤的胆固醇卵磷脂缓释小丸中，评价了肝素结合的EGF样生长因子对烧伤创面愈合的影响。分别于烧伤后第3、5、10天处死实验组(肝素结合EGF样生长因子治疗)和对照组(未治疗)小鼠。测定每个创面的总烧伤面积、组织学、角质形成细胞增殖和转化生长因子-α的原位杂交分析。伤后第5天，实验组创面平均面积为1.07 cm(2)，对照组为2.20 cm(2)(P=0.04)。5-溴-2-脱氧尿苷免疫组织化学检测显示，烧伤后第5天，实验组边缘角质形成细胞和毛囊上皮细胞增殖指数明显高于对照组。原位杂交结果显示，烫伤后第5天，实验动物肺组织中转化生长因子-α基因表达上调。局部应用肝素结合的EGF样生长因子可显著加速小鼠深Ⅱ度烧伤的再上皮化，增加角质形成细胞的增殖活性，并增强内源性转化生长因子-αmRNA的产生。
Heparin-binding EGF-like growth factor has been identified in human burn-wound fluid and in the epithelial cells of excised human partial-thickness burns. In the present study, the effect of heparin-binding EGF-like growth factor on burn-wound healing was evaluated by incorporating purified, recombinant heparin-binding EGF-Like growth factor into slow-release cholesterol-lecithin pellets that were applied topically to partial-thickness burns in mice. Both experimental (heparin-binding EGF-like growth factor-treated) and control (untreated) mice were sacrificed on days 3, 5, and 10 after burn. Total burn-wound area, histology, keratinocyte proliferation, and in situ hybridization analysis for transforming growth factor-a were determined for each wound. The mean wound area of the experimental group on day 5 after burn was 1.07 cm(2), compared with 2.20 cm(2) for controls (P = 0.04). Cellular proliferation las measured by immunohistochemical detection of 5-Bromo-2-deoxyuridine) on day 5 after burn in marginal keratinocytes and follicular epithelial cells was greater in the experimental group than in the control group. In situ hybridization showed up-regulation of transforming growth factor-alpha mRNA levels in experimental animals by day 5 after burn. Topical application of heparin-binding EGF-like growth factor significantly accelerates the reepithelialization of murine partial-thickness burns, increases keratinocyte proliferative activity, and enhances production of endogenous transforming growth factor-alpha mRNA.