Animal Cell Technology: Basic & Applied Aspects
Animal Cell Technology: Basic & Applied Aspects
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动物细胞技术：基础
DOI：
10.1007/0-306-46865-4
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发表时间：
2002
影响因子：
2.9
通讯作者：
Haiime
中科院分区：
文献类型：
--
作者：
Alan Moore;Tadao Ohno;Ralf Portner;Ryuzo Sasaki;Kazuo Shin;Haiime
为了研究神经节苷类在突触活性中的作用，我们利用神经酰胺类似物D-PDMP及其对映体L-PDMP抑制和刺激培养皮层神经元神经节苷类的生物合成。长期使用两种PDMP异构体对神经元间细胞内自发同步振荡活动测量的功能性突触形成表现出相反的影响：D-PDMP抑制和L-PDMP促进。L-PDMP对突触活性的上调可能与丝裂原活化蛋白激酶缓慢但强劲的激活有关。L-PDMP治疗大鼠短暂性前脑缺血后8臂迷宫任务中习得的空间记忆缺失，为神经退行性疾病的治疗提供了一种新的潜在方法。
To address the role of brain gangliosides in synaptic activity, the ceramide analogs, Dthreo-1-phenyl-2-decanoylamino-3-morpholino-l-propanol (D-PDMP) and its enantiomer, L-PDMP, were used to inhibit and stimulate ganglioside biosynthesis in cultured cortical neurons. Prolonged treatment with both PDMP isomers exhibited opposite effects on functional synapse formation measured by spontaneous synchronized oscillatory activity of intracellular between the neurons: suppression by D-PDMP and facilitation by L-PDMP. Up-regulation of synaptic activity by L-PDMP could be correlated with the slow but robust activation of mitogen-activated protein kinase. Treatment with L-PDMP after transient forebrain ischemia in rats ameliorated the deficit of a well-learned spatial memory by an 8-arm maze task, suggesting a new potential therapeutic approach for neurodegenerative disorders.
海胆卵胶衣中含聚唾液酸糖蛋白的鉴定
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kitazume,S;Kitajima,K;Inoue,S;Troy2nd,FA;Cho,JW;Lennarz,WJ;Inoue,Y
通讯作者：
Inoue,Y