Cas12a-Activated Universal Field-Deployable Detectors for Bacterial Diagnostics
Cas12a-Activated Universal Field-Deployable Detectors for Bacterial Diagnostics
复制标题
用于细菌诊断的 Cas12a 激活通用现场部署探测器
DOI：
10.1021/acsomega.0c01911
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发表时间：
2020-06-23
期刊：
影响因子：
4.1
通讯作者：
Li, Jinghong
中科院分区：
文献类型：
--
作者：
Sun, Yun;Liu, Hong;Li, Jinghong
Field-deployable detectors of disease biomarkers provide a simple and fast analysis of clinical specimens. However, most of the existing field-deployable diagnostics have poor sensitivity and are not suitable for the detection of biomarkers with low abundance. Herein, we report a highly sensitive and rapid colorimetric readout paper-based assay for pathogen detection by integrating the unique collateral activity of a Cas12a-activated universal field-deployable detector (CUFD). The collateral effect of Cas12a results in a nonspecific destruction of a fluorophore biotin-labeled ssDNA reporter for the CUFD. This technique can quantify seven different kinds of pathogens in blood samples without any purification procedure, with sensitivity as low as 10 aM for the Shigella dysenteriae DNA. This CUFD technique has significant potential for the detection of pathogenic DNA as well as other types of DNA or RNA targets at the point-of-care application.
