Liver response to indomethacin-induced intestinal injury.
Liver response to indomethacin-induced intestinal injury.
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肝脏对吲哚美辛引起的肠道损伤的反应。
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发表时间：
2002
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影响因子：
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通讯作者：
P. Kohout
中科院分区：
文献类型：
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作者：
Z. Červinková;Dagmar Radvaková;P. Kohout
本研究旨在探讨消炎痛致小鼠小肠屏障功能受损对肝损伤生化指标(血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、胆红素)和肝功能参数(血清白蛋白浓度、肝脏DNA合成)的影响。两次皮下注射吲哚美辛(Sigma)7.5 mg/kg，间隔24小时，分别于第二次注射后24、48、72小时处死大鼠。对照组大鼠以同样的方式注射消炎痛(5%NaHCO3，pH 7.4，1.0ml/kg)。小肠损伤通过增加通透性(以乳果糖-甘露醇指数测量)来证实。在第二次给药后48(p<；0.01)和72(p<；0.05)小时，消炎痛处理的大鼠小肠DNA合成显著增加(用~3H-胸腺嘧啶核苷掺入法估计)，证明了修复过程的诱导。消炎痛处理组大鼠肝损伤的所有生化指标在所有记录时间段均显著降低(p<；0.05)。相反，消炎痛治疗组大鼠的血清白蛋白浓度在所有时间段均显著降低(p<；0.01)，白蛋白浓度是肝功能的预测指标。为了解释消炎痛引起的小肠屏障功能受损对肝脏的这些相反的结果，值得进一步研究。
The aim of our study was to evaluate the impact of impaired barrier function of the small intestine induced by indomethacin on biochemical markers of liver damage (serum levels of alanine aminotransferase, aspartate aminotransferase, bilirubin), and liver functional parameters (serum concentration of albumin, liver DNA synthesis). Indomethacin (Sigma) was administered in 2 injections in a dose of 7.5 mg/kg subcutaneously spaced 24 hours apart, rats were sacrificed 24, 48 or 72 hours after the second dose of indomethacin. Control rats received indomethacin vehicle (5% NaHCO3, pH 7.4, 1.0 ml/kg) in the same manner. Small intestine injury was approved by increased permeability (measured as a lactulose-mannitol index). Significant increase of small intestine DNA synthesis (estimated by incorporation of 3H thymidine) in indomethacin-treated rats 48 (p < 0.01) and 72 (p < 0.05) hours after the second dose of indomethacin documents induction of reparative process. All biochemical markers of liver injury were significantly decreased in indomethacin treated rats in all recorded intervals (p < 0.05). By contraries, serum concentration of albumin, which predicates about liver function, was in indomethacin-treated rats significantly decreased in all intervals (p < 0.01). To explain these contrarious results of indomethacin-induced impaired barrier function of the small intestine on the liver deserves further studies.