Intracisternal DIDS enhances ventilatory response to rebreathing CO2 in rats.
Intracisternal DIDS enhances ventilatory response to rebreathing CO2 in rats.
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脑池内 DIDS 增强大鼠对再呼吸 CO2 的通气反应。
DOI：
10.1152/jappl.1988.65.4.1611
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Johnson,NL
中科院分区：
文献类型：
--
作者：
Adams,JM;Johnson,NL
在11只麻醉大鼠中，我们通过将模拟脑脊液（CSF）中的转运抑制剂4，4 '-二异硫氰基芪-2，2'-二磺酸（DIDS）注入小脑延髓池，验证了载体介导的阴离子转运部分决定了延髓对急性高碳酸血症的化学感受器反应的假设。在另外5只接受假CSF输注的大鼠中，我们发现假CSF对再呼吸CO2的反应没有影响。注入池的染料染色的假定化学感受器领域的腹侧表面的髓质。10至1,000 nM的DIDS以剂量相关的方式增加了对CO2的呼吸反应，但对动脉压或心率没有影响。在1,000 nM时，由于呼吸量和呼吸速率，高碳酸血症分钟通气反应几乎加倍。我们的结论是，净效应的阴离子运输是减轻刺激的髓质化学感受器在急性高碳酸血症。
In 11 anesthetized rats, we tested the hypothesis that carrier-mediated anion transport in part determines the medullary chemoreceptor response to acute hypercapnia by infusing the transport inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) in mock cerebrospinal fluid (CSF) into the cisterna magna. In five additional rats with sham CSF infusion, we found no effect of mock CSF on the response to rebreathing CO2. Dye infused into the cistern stained the putative chemoreceptor areas on the ventral surface of the medulla. DIDS, at 10 to 1,000 nM, increased the respiratory response to CO2 in a dose-related manner but had no effect on arterial pressure or heart rate. At 1,000 nM, the hypercapnic minute ventilation response was almost doubled because of both volume and rate of breathing. We conclude that the net effect of anion transport is to mitigate the stimulus to the medullary chemoreceptors during acute hypercapnia.