Effects of the consecutive cultivation and periodic residue incorporation of Bacillus thuringiensis (Bt) cotton on soil microbe-mediated enzymatic properties
Effects of the consecutive cultivation and periodic residue incorporation of Bacillus thuringiensis (Bt) cotton on soil microbe-mediated enzymatic properties
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DOI：
10.1016/j.agee.2017.01.017
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发表时间：
2017-02-15
影响因子：
6.6
通讯作者：
Cui, Jinjie
中科院分区：
文献类型：
--
作者：
Chen, Zhenhua;Wei, Kai;Cui, Jinjie
苏云金芽孢杆菌（Bt）棉花杀虫Cry蛋白在土壤中的风险评估主要基于微观和短期实验。缺乏长期的田间结果，特别是当Bt棉残留物被掺入土壤时。本研究通过为期6年的田间试验，研究了不同种植年限和不同Cry 1Ac输入方式对Bt棉Cry 1Ac蛋白和土壤微生物介导的酶活性的影响。结果表明，转Bt基因抗虫棉品种ZM 41的Cry 1Ac蛋白的持久性随栽培时间的延长和定期残留物掺入而增加。此外，时间残留掺入有一个相对较大的贡献，Cry 1Ac蛋白在土壤中的持久性比他们在生长期释放。无论是Bt棉种植还是秸秆还田，土壤微生物生物量均受到显著抑制。转Bt基因棉对脱氢酶活性有明显的促进作用，而残留物掺入则抑制了脱氢酶活性。Bt-棉株还田对土壤中β-葡萄糖苷酶、硝酸还原酶、磷酸单酯酶和芳基硫酸酯酶的活性有明显的促进作用。基于结构方程模型分析，这四种酶的酶活性的变化归因于来自Cry 1Ac蛋白的直接作用和通过脱氢酶的间接作用。(C)2017爱思唯尔B. V.保留所有权利。
Risk assessments of insecticidal Cry proteins from Bacillus thuringiensis (Bt) cotton in soils have been mainly based on microcosm and short-term experiments. There is a lack of longer-term field results, especially when Bt-cotton residues are incorporated into the soil. In this study, we used a six-year field trial to evaluate how cultivation duration and Cry1Ac-inputted modes of Bt-cotton affect the persistence of Cry1Ac proteins and soil microbe-mediated enzymatic properties. The results showed that the persistence of Cry1Ac proteins increased with cultivation duration and periodic residue incorporation of the transgenic Bt-cotton variety ZM41. Moreover, temporal residue incorporation had a relatively larger contribution to the persistence of Cry1Ac proteins in the soil than their release in the growth period. Regardless of Bt-cotton cultivation or residue incorporation, soil microbial biomass was significantly suppressed. However, the dehydrogenase activity was significantly stimulated in Bt-cotton cultivation but suppressed in residue incorporation. The activities of beta-glucosidase, nitrate reductase, phosphomonoesterase and arylsulfatase were significantly stimulated in soils with Bt-cotton residue incorporation. Based on a structural equation model analysis, the change in enzymatic activity of these four enzymes was attributed to both a direct effect from Cry1Ac proteins and an indirect effect via dehydrogenase. (C) 2017 Elsevier B.V. All rights reserved.