PRODUCTION OF A MOUSE MONOCLONAL-ANTIBODY REACTIVE WITH A HUMAN NUCLEAR ANTIGEN ASSOCIATED WITH CELL-PROLIFERATION
PRODUCTION OF A MOUSE MONOCLONAL-ANTIBODY REACTIVE WITH A HUMAN NUCLEAR ANTIGEN ASSOCIATED WITH CELL-PROLIFERATION
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DOI：
10.1002/ijc.2910310104
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发表时间：
1983-01-01
影响因子：
6.4
通讯作者：
STEIN, H
中科院分区：
文献类型：
--
作者：
GERDES, J;SCHWAB, U;STEIN, H
描述了小鼠单克隆抗体 Ki-67 的生产。 Ki-67 抗体识别增殖细胞中存在的核抗原，但静息细胞中不存在。 Ki-67 免疫染色显示皮质滤泡生发中心细胞、皮质胸腺细胞、胃肠粘膜颈部细胞、未分化精原细胞和许多人类细胞系的细胞具有核反应性。 Ki-67抗体不与已知处于静息期的细胞发生反应，如胃肠粘膜的淋巴细胞、单核细胞、壁细胞和潘氏细胞、肝细胞、肾细胞、成熟精子细胞、脑细胞等。用Ki-67抗体刺激外周血淋巴细胞后，可诱导Ki-67识别的抗原表达。 植物血凝素，而在佛波酯刺激的人类白血病 HL-60 细胞中，它在静息阶段分化为成熟巨噬细胞时消失。 Ki-67 显然是针对与细胞增殖相关的核抗原。肿瘤活检的第一系列免疫染色表明，Ki-67 可能是一种有效的工具，可以轻松快速地评估肿瘤中增殖细胞的比例。
The production of a mouse monoclonal antibody, Ki-67, is described. The Ki-67 antibody recognized a nuclear antigen present in proliferating cells, but absent in resting cells. Immunostainings with Ki-67 revealed nuclear reactivity in cells of germinal centers of cortical follicles, cortical thymocytes, neck cells of gastrointestinal mucosa, undifferentiated spermatogonia and cells of a number of human cell lines. The Ki-67 antibody did not react with cells known to be in a resting stage, such as lymphocytes, monocytes, parietal cells and Paneths cells of gastrointestinal mucosa, hepatocytes, renal cells, mature sperm cells, brain cells, etc. Expression of the antigen recognized by Ki-67 could be induced in peripheral blood lymphocytes after stimulation with phytohemagglutinin, whereas it disappeared from human leukemia HL-60 cells stimulated with phorbol esters to differentiate into mature macrophages in a resting stage. Ki-67 evidently is directed against a nuclear antigen associated with cell proliferation. A first series of immunostainings of tumor biopsies indicated that Ki-67 may be a potent tool for easy and quick evaluation of the proportion of proliferating cells in a tumor.