Application of WGA lectin staining for visualization of the connective tissue in skeletal muscle, bone, and ligament/tendon studies.
Application of WGA lectin staining for visualization of the connective tissue in skeletal muscle, bone, and ligament/tendon studies.
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DOI：
10.1002/jemt.20865
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发表时间：
2011-01
影响因子：
2.5
通讯作者：
Kostrominova, Tatiana Y.
中科院分区：
文献类型：
--
作者：
Kostrominova, Tatiana Y.
在使用单克隆和多克隆抗体对组织切片中的特定蛋白进行免疫染色期间，一般组织染色/背景或主要结构特征的可视化有助于精确定位感兴趣的蛋白。在骨骼肌研究中，通常使用抗结缔组织或质膜蛋白（胶原蛋白1，层粘连蛋白，小窝蛋白3）的抗体进行免疫染色。虽然这些蛋白质的免疫染色效果很好，但它耗时、昂贵，限制了可以在单个切片上使用的针对感兴趣蛋白质的抗体的数量，并且不适用于某些染色技术。凝集素在早期的出版物中经常用于骨骼肌纤维边界和结缔组织可视化，但在当前的研究中并不常见。本文探讨了共染色的肌肉，骨骼，韧带和肌腱组织切片与荧光标记的麦胚凝集素（WGA）凝集素作为一种工具，用于可视化的结缔组织。当前研究的结果表明，荧光WGA凝集素共染色是一种具有成本效益的，快速和方便的结缔组织可视化方法，特别是在大量洗涤减少结构染色的研究中，这些结构是研究的主要兴趣。
During immunostaining of specific proteins in tissue sections using monoclonal and polyclonal antibodies visualization of general tissue staining/background or major structural features is helpful to pinpoint precise localization of the protein of interest. Often in skeletal muscle research immunostaining with antibodies against connective tissue or plasma membrane proteins (collagen 1, laminin, caveolin 3) are used for this purpose. Although immunostaining for these proteins works well, it is time consuming, costly, limits the number of antibodies against protein of interest that can be used on a single section, and is not applicable to some staining techniques. Lectins were frequently used in earlier publications for skeletal muscle fiber boundaries and connective tissue visualization, but are not common in the current research studies. The present paper investigates co-staining of muscle, bone, ligament and tendon tissue sections with fluorescently tagged wheat germ agglutinin (WGA) lectin as a tool for visualization of connective tissue. The results of the current study show that fluorescent WGA lectin co-staining is a cost-effective, fast and convenient method for connective tissue visualization, especially in the studies where extensive washes reduce staining of the structures that are the primary interest of the investigation.
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影响因子：
4.4
作者：
DIIORIO, G;COTRUFO, R
通讯作者：
COTRUFO, R
影响因子：
1.6
作者：
Mozdziak, PE;Fassel, TA;Cassens, RG
通讯作者：
Cassens, RG
影响因子：
3.2
作者：
Mundegar, Rustam R.;Franke, Elke;Wernig, Anton
通讯作者：
Wernig, Anton
影响因子：
3.6
作者：
Kostrominova, Tatiana Y.;Pasyk, Krystyna A.;Faulkner, John A.
通讯作者：
Faulkner, John A.
DOI：
10.1007/s00438-009-0460-8
发表时间：
2009-07
期刊：
Molecular genetics and genomics : MGG
影响因子：
--
作者：
De Hoff PL;Brill LM;Hirsch AM
通讯作者：
Hirsch AM