Exogenous application of L‐histidine suppresses bacterial diseases and enhances ethylene production in rice seedlings
Exogenous application of L‐histidine suppresses bacterial diseases and enhances ethylene production in rice seedlings
复制标题
外源施用L-组氨酸抑制细菌性病害并提高水稻幼苗乙烯产量
DOI：
10.1111/ppa.13037
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发表时间：
2019
期刊：
影响因子：
2.7
通讯作者：
Takahashi H.
中科院分区：
文献类型：
--
作者：
Yariyama S.;Ando S.;Seo S.;Nakaho K.;Miyashita S.;Kanayama Y.;Takahashi H.
外源应用全组氨酸通过激活乙烯（ET）依赖性信号通路增强番茄（Solanum lycopersicum）和拟南芥（arabidopsis thaliana）对病原体的抗性。本研究研究了全组氨酸对水稻幼苗细菌性病害的抑制作用。水稻种子在28℃下分别用10 mm组氨酸、10 mm赖氨酸或蒸馏水（DW）浸泡48 h，以刺激萌发。处理后的种子真空接种伯克霍尔德菌。plantarii。组氨酸处理能抑制这两种病原菌引起的幼苗病害，而赖氨酸和DW处理则不能。用1 -组氨酸处理种子可诱导ET应答性防御相关基因OsGLP8 - 12的表达。由于真空接种后用1 -组氨酸处理水稻幼苗的病害未被抑制，因此在接种前用1 -组氨酸预处理水稻幼苗可能会激活植物免疫系统。事实上，在1 -组氨酸处理的水稻幼苗中，乙烯产量和1 -氨基环丙烷- 1 -羧酸（ACC）合成酶2 （OsACS2）转录物的丰度增加，而在DW处理的水稻幼苗中则没有。此外，用乙烯前体ACC处理水稻种子，抑制了由b菌引起的水稻菌苗腐病。与1 -组氨酸处理相比，用氨基乙酸（ACC合成酶的抑制剂）处理水稻种子会部分削弱对疾病的抑制作用。综上所述，l -组氨酸似乎通过乙烯依赖的抗性途径抑制水稻细菌性幼苗疾病。
Exogenous application ofl‐histidine enhances resistance to pathogens in tomato (Solanum lycopersicum) andArabidopsis thalianavia activation of the ethylene (ET)‐dependent signalling pathway. In this study, the efficacy ofl‐histidine for suppression of bacterial diseases in rice seedlings was investigated. Rice seeds were soaked in 10 mm l‐histidine, 10 mm l‐lysine, or distilled water (DW) as a control for 48 h at 28 °C to stimulate germination. Treated seeds were then vacuum‐inoculated withBurkholderia glumaeorB. plantarii. Seedling diseases caused by both of these bacterial pathogens were suppressed by treatment withl‐histidine but not by treatment withl‐lysine or DW. Expression of an ET‐responsive defence‐related gene,OsGLP8‐12, was induced by treatment of seeds withl‐histidine. As diseases were not suppressed in rice seedlings treated withl‐histidine after vacuum‐inoculation, pretreatment of rice seedlings withl‐histidine before inoculation might activate the plant immune system. Indeed, ethylene production and the abundance of 1‐aminocyclopropane‐1‐carboxylic acid (ACC) synthase 2 (OsACS2) transcript increased in healthy seedlings grown from rice seeds treated withl‐histidine but not in those treated with DW. Furthermore, treatment of rice seeds with ACC, an ethylene precursor, suppressed bacterial rice seedling rot caused byB. glumaeas effectively as did treatment withl‐histidine, whereas treatment of rice seeds with aminooxyacetic acid, an inhibitor of ACC synthase, partially compromised disease suppression. Taken together,l‐histidine seems to suppress bacterial rice seedling diseases via an ethylene‐dependent resistance pathway.